Međudjelovanje kromatin remodelirajućih kompleksa RSC i ISW1 i histon-metiltransferaze Set1 u procesu aktivacije transkripcije gena PHO5 i PHO8 kvasca Saccharomyces cerevisiae

master's thesis

• Title (english): Interplay between chromatin remodeling complexes RSC and ISW1 and histonemethyltransferase Set1 in regulation of transcription activation of Saccharomyces cerevisiae genes PHO5 and PHO8
• Author: Lovro Jalšić
• Mentor: Slobodan Barbarić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Biochemistry); Zagreb
• Defense date and country: 2018-10-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Promotor gena PHO5 kvasca Saccharomyces cerevisiae jedan je od najbolje istraženih modela za proučavanje mehanizma remodeliranja strukture kromatina pri aktivaciji transkripcije. U prethodnim istraživanjima je utvrđena esencijalna uloga remodelirajućeg kompleksa RSC u procesu aktivacije promotora gena PHO5. Također je pokazano represivno djelovanje metilacije katalizirane histon-metiltransferazom Set1 u procesu remodeliranja promotora PHO5. U ovom radu utvrđeno je antagonističko međudjelovanje kompleksa RSC i enzima Set1 pri aktivaciji promotora PHO5, dok inaktivacija enzima Set1 i kompleksa RSC, pojedinačno i zajedno nema utjecaj na proces remodeliranja promotora PHO8. Također je pokazano međudjelovanje kompleksa RSC i ISW1 pri aktivaciji promotora PHO5, pri čemu ova dva kompleksa mogu, ovisno o koncentraciji kompleksa RSC u stanici, djelovati sinergistički i antagonistički pri aktivaciji transkripcije gena PHO5.

Abstract (english)

The Saccharomyces cerevisiae PHO5 promoter is one of the best explored model systems for research of transcription activation trough chromatin remodeling. Previous findings established the RSC complex as the only chromatin remodeling complex essential for PHO5 promoter remodeling. Previous findings have also shown that histone methylation catalyzed by histone-methyltransferase Set1 has a repressive role in chromatin remodeling at the PHO5 promoter. Here we show an antagonistic relationship between the RSC complex and enzyme Set1 in PHO5 promoter activation, while both individual and simultaneous inactivation of the RSC complex and Set1 enzyme does not have any effect on PHO8 promoter remodeling. Here we also show that complexes RSC and ISW1 display both a synergistic or antagonistic relationship in PHO5 promoter activation, depending on the concentration of active RSC complex in cells.

• Keywords: remodeliranje kromatina; promotor PHO5; RSC; ISW1; Set1; kvasac Saccharomyces cerevisiae
• Keywords (english): chromatin structure remodeling; promoter PHO5; RSC; ISW1; Set1; yeast Saccharomyces cerevisiae
• Language: croatian
• URN:NBN: urn:nbn:hr:159:719996
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-11-14 11:48:05