Primjena eutektičkih otapala i novih tehnologija u izolaciji lignana i fenolnih kiselina iz pogače lana

master's thesis

• Title (english): Application of eutectic solvents and new technologies in isolation of lignans and phenolic acids from flaxseed cake
• Author: Mia Ivanov
• Mentor: Marko Obranović (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Oil and Fat Technology); Zagreb
• Defense date and country: 2018-07-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Lanena pogača, kao nusproizvod proizvodnje lanenog ulja, vrlo je bogata nutritivno vrijednim polifenolnim spojevima, što ju čini odličnom sirovinom za njihovu ekstrakciju. Do sada korištene metode izolacije su vremenski zahtjevne i uz značajnu upotrebu organskih otapala. Cilj ovog rada bio je zamijeniti organska otapala s ekološki prihvatljivim eutektičnim otapalima te ubrzati proces ekstrakcije fenolnih spojeva iz pogače lana koristeći ultrazvučno-mikrovalni ekstraktor. Od tri istraživana eutektička otapala najpogodnijim se pokazao kolin klorid:etilen glikol (ChClEg). Najpogodnijim tretmanom za izdvajanje fenolnih spojeva pokazao se tretman snage 300 W u trajanju 9 minuta uz dodatak 40 % 0,1 M NaOH. Pri optimalnim uvjetima izolacije dobivene su koncentracije fenolnih spojeva: sekoizolarikirezinol-diglukozid (6,55 mg g-1), glukozid p-kumarinske kiseline (8,50 mg g-1) i glukozid ferulinske kiseline (3,39 mg g-1). Na taj način je uspješno ekstrahirano 48 % koncentracije glukozida p-kumarinske kiseline, 48 % koncentracije glukozida ferulinske kiseline i 39 % koncentracije sekoisolarikirezinol diglukozida, u odnosu na referentnu metodu.

Abstract (english)

Flaxseed cake, a by-product of flaxseed oil production, is very rich in bioactive polyphenolic compounds, and is therefore a good raw material for their extraction. Standard isolation methods are time-consuming acquiring considerable amounts of organic solvents. The aim of this paper was to replace organic solvents with ecologically acceptable eutectic solvents and to speed up the extraction process of phenolic compounds from flaxseed using an ultrasonic-microwave extractor. Out of the three tested eutectic solvents, the preferred one was choline chloride: ethylene glycol (ChClEg). Optimum treatment for the isolation of phenolic compounds was at 300 W power for a period of 9 minutes with the addition of 40 % 0.1 M NaOH. At these optimal isolation conditions, obtained concentrations of phenolic compounds were secoizolariciresinol-diglucoside (6.55 mg g-1), p-coumaric acid glucoside (8.50 mg g-1) and ferulic acid glucoside (3.39 mg g-1). Thus 48 % of p-coumaric acid glucose concentrations, 48 % ferulic acid glucose concentration and 39 % secoisolariciresinol diglucoside concentration were successfully extracted, compared to the reference method.

• Keywords: lanena pogača; lignani; fenolne kiseline; eutektička otapala; ultrazvučno-mikrovalna ekstrakcija
• Keywords (english): flaxseed cake; lignans; phenolic acids; deep eutectic solvents; ultrasonic – microwave assisted extraction
• Language: croatian
• URN:NBN: urn:nbn:hr:159:824229
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-11-28 10:34:56