Određivanje aminoglikozida u mlijeku LC-MS/MS metodom

master's thesis

• Title (english): Determination of aminoglycosides in milk by LC-MS/MS
• Author: Liridone Fejzuli
• Mentor: Blaženka Kos (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2017-11-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Interdisciplinary Technical Sciences

Abstract

Aminoglikozidni antibiotici se upotrebljavaju za kontrolu bolesti, kao aditivi za hranu, ili sintetski promotori rasta unutar različitih sektora industrije (stočarstvo, akvakultura i poljoprivreda) kako bi se poboljšalo iskorištenje hrane za životinje te poticao rast životinja. Veliki udio apliciranih veterinarskih antibiotika se izlučuje kroz urin, izmet, mlijeko i jaja kao nemetabolizirani ishodišni spojevi, ili se akumuliraju unutar različitih tkiva. To može predstavljati stvarnu prijetnju potrošaču, bilo kroz izloženost rezidualnim ostacima antibiotika, prijenos antibiotičke rezistencije ili povišene stope alergija koje su posljedica prisutnosti antibiotika u hrani. Negativni aspekti uporabe antibiotika u prehrani životinja potvrđuju važnost razvoja učinkovitih analitičkih tehnika za utvrđivanje njihove prisutnosti u proizvodima namijenjenim životinjama te stupnja bioakumulacije u tkivima. Stoga je cilj ovog rada determinacija i kvantifikacija aminoglikozida u uzorcima mlijeka. Ispitivani analiti su ekstrahirani kroz čvrstu fazu (SPE) te su određeni uz pomoć tekućinske kromatografije - tandem masene spektrometrije (LC-MS/MS). Metoda je validirana sukladno Odluci Komisije 2002/657/EZ Europske unije te je računalnim programom instrumenta MassHunter Quantitative analysis for QQQ version B.07.00., primijenjena za analizu aminoglikozida u uzorcima mlijeka sakupljenih iz različitih dijelova Hrvatske.

Abstract (english)

Aminoglycosides are used for disease control, as feed additives, or as synthetic growth promoters within various sectors (livestock farming, aquaculture and agricultural activities) in order to to improve feed utilization and animal production. A high proportion of antibiotics are excreted via urine, feces, milk and eggs as the non-metabolized parent compounds, or accumulate in a high concentration in various tissues. This may pose a real threat to the consumer, either through exposure to the residues, the transfer of antibiotic resistance or increased allergies resulting from antibiotic presence in foods. The negative aspects of the use of antibiotics in animal diets confirm the importance of the development of effective analytical techniques to determine the presence of antibiotics or growth promoters in animal products and the level of bioaccumulation in tissues and in food products. The aim of this study was the determination and quantification of aminoglycosides in samples of milk. For all samples the same procedure was used, so the questioned analytes were extracted threw solid phase extraction (SPE) and were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method has been validated, in accordance with the EU Commission Decision 2002/657/EC and was, threw the use of a softwer program of the instrument MassHunter Quantitative analysis for QQQ version B.07.00, applied to the analysis of aminoglycosides in samples of milk collected from different parts of Croatia.

• Keywords: aminoglikozidi; LC-MS/MS; rezidui; kvantifikacija
• Keywords (english): aminoglycosides; LC-MS/MS; residues; quantification
• Language: croatian
• URN:NBN: urn:nbn:hr:159:964308
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-12-03 10:21:30