Učinak resveratrola na PCB 77 induciranu proliferaciju u kulturama stanica reprodukcijskog sustava

master's thesis

• Title (english): Effect of resveratrol on PCB 77 induced proliferation in cell cultures of reproductive system
• Author: Kristina Koropatnicki
• Mentor: Ivana Kmetič (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2017-12-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu je ispitan učinak resveratrola na PCB 77 induciranu proliferaciju u kulturama stanica reprodukcijskog sustava. U tu svrhu koristile su se stanice ovarija kineskog hrčka CHO-K1 i ljudske stanice raka dojke MCF-7. Utjecaj samog resveratrola (5 – 100 μM) na CHO-K1 i MCF-7 stanice ispitan je nakon 48h inkubacije metodom MTT. U obje stanične linije statistički značajan inhibitorni/citotoksični učinak na proliferaciju imale su koncentracije resveratrola od 75 i 100 μM te su upravo stoga u daljnjim pokusima korištene koncentracije resveratrola niže od 75 μM. Utjecaj PCB 77 (niske doze: 0,1 – 10 μM) na proliferaciju stanica ispitan je metodom Trypan Blue nakon 24, 48 i 72h inkubacije. Niske doze PCB 77 (do 5 μM) su inducirale proliferaciju, a najveća indukcija stanične proliferacije zabilježena je nakon 72 sata pri koncentracijama PCB 77 od 1 i 5 μM na CHO K1 staničnoj liniji te pri 0,5 i 1 μM na MCF-7 kulturi stanica. U zadnjoj fazi istraživanja obje kulture stanica su preinkubirane resveratrolom a zatim tretirane s 1 μM PCB 77. Resveratrol je efektivno suprimirao PCB-em stimuliranu proliferaciju stanica s izraženijim učinkom u staničnoj liniji kancera dojke.

Abstract (english)

In this study the effect of resveratrol on PCB 77 induced proliferation in cell culture of the reproductive system was tested. Chinese hamster ovary cells CHO-K1 and MCF-7 human breast cancer cells were used for that purpose. Effect of resveratrol itself (5 – 100 μM) on CHO-K1 and MCF-7 cell cultures has been tested after 48 h incubation by MTT method. In both cell lines statistically significant inhibiting/cytotoxic effect on proliferation had the concentration of resveratrol of 75 and 100 μM and therefore, in further experiments, the applied concentration of resveratrol was lower than 75 μM. The effect of PCB 77 (low doses: 0,1 – 10 μM) on cell prolifetarion was tested by Trypan Blue method after 24, 48 and 72 h incubation. Low doses of PCB 77 (up to 5 μM) induced proliferation and the largest induction of cell proliferation was observed after 72 hours at PCB 77 concentration of 1 and 5 μM in the CHO-K1 cell line and at 0,5 and 1 μM in MCF-7 cell culture. In the last phase of the study, both cell cultures were pre-incubated with resveratrol and then treated with 1 μM PCB 77. Resveratrol effectively suppressed PCB stimulated proliferation of cells with more pronounced effect in the breast cancer cell line.

• Keywords: resveratrol; PCB 77; MFC-7 stanična linija; CHO-K1 stanična linija; proliferacija
• Keywords (english): resveratrol; PCB 77; CHO-K1 cell line; MCF-7 cell line; proliferation
• Language: croatian
• URN:NBN: urn:nbn:hr:159:890653
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-12-03 10:27:08