Uzgoj kvasca Scheffersomyces stipitis na laboratorijskoj tresilici i u laboratorijskom bioreaktoru s ciljem proizvodnje (S)-adenozil-L- metionina

master's thesis

• Title (english): Cultivation of yeast Scheffersomyces stipitis in laboratory shaker and in laboratory bioreactor with purpose of (S)-adenosyl-L-methionine production
• Author: Elizabeta Kralj
• Mentor: Damir Stanzer (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Fermentation and Yeast Technology); Zagreb
• Defense date and country: 2016-07-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

(S)-adenozil-L-metionin (SAMe, AdoMet) je glavni donor metilne skupine u reakcijama kataliziranim enzimima metiltransferazama te sudjeluje u metilaciji DNA, RNA, sterola i fosfolipida. Kod ljudi se poremećaji u metabolizmu SAMe povezuju s različitim oboljenjima pa ima značajnu ulogu kao farmaceutik i nutraceutik u liječenju depresije, osteoartritisa, bolesti jetre i Alzheimerove bolesti. Najčešće se proizvodi mikrobiološkom sintezom pomoću kvasaca. U ovom radu uzgajana je biomasa soja M12 kvasca Scheffersomyces stipitis na laboratorijskoj tresilici i u laboratorijskom bioreaktoru. S ciljem ispitivanja utjecaja dodatka protektivnih spojeva (glicerola, mioinozitola i kolina) na rast biomase i udio SAMe uzgoji su provedeni u tzv. O-podlozi bez i uz dodatak protektivnih spojeva. Dodatak glicerola u O-podlogu u uvjetima povećane aeracije pozitivno je djelovao na rast soja M12 kao i na udio SAMe u kvascu uzgojenom na laboratorijskoj tresilici. Uzgojem soja M12 kvasca S. stipitis u laboratorijskom bioreaktoru u modificiranoj O-podlozi s glicerolom kao osnovnim izvorom ugljika postignut je dobar rast soja M12 kvasca S. stipitis, a uz pulsni dodatak D,L-metionina na početku stacionarne faze ostvareno je povećanje udjela SAMe u kvaščevoj biomasi.

Abstract (english)

(S)-adenosyl-L-methionine (SAMe, AdoMet) is a primary donor of methyl group in reactions catalyzed by methyltransferases and is directly involved in methylation of DNA, RNA, sterols and phospholipids. Disorders in SAMe metabolism are associated with various diseases in humans, so it has an important role as a pharmaceutical and a nutraceutical in treatments of conditions such as depression, osteoarthritis, liver diseases and Alzheimer's disease. SAMe is most commonly produced by microbial synthesis using yeasts. In this study a biomass of yeast strain Scheffersomyces stipitis M12 was grown in laboratory shaker and in laboratory bioreactor. Cultivations were carried out in O-medium without and with addition of protective compounds, so the impact of this compounds (glycerol, myo-inositol and choline) on the growth of biomass and on the SAMe content could have been tested. Addition of glycerol in O-medium in conditions of increased aeration positively affected the growth of M12 strain as well as SAMe content in the laboratory shaker. During the cultivation of M12 strain in a laboratory bioreactor in the modified O-medium with glycerol as a primary carbon source a satisfactory amount of M12 biomass was produced, while pulsed addition of D, L-methionine at the start of the stationary phase increased the SAMe content in biomass.

• Keywords: (S)-adenozil-L-metionin; kvasac Scheffersomyces stipitis; glicerol; bioreaktor
• Keywords (english): (S)-adenosyl-L-methionine; yeast Scheffersomyces stipitis; glycerol; bioreactor
• Language: croatian
• URN:NBN: urn:nbn:hr:159:034586
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-26 11:11:32