Detekcija i karakterizacija trim mobilnih elemenata u vrstama školjkaša cerastoderma edule i Glycymeris glycymeris

master's thesis

• Title (english): Detection and characterization of trim mobile elements in bivalve SPECIES Cerastoderma edule and Glycymeris glycymeris
• Author: Andrea Cedilak
• Mentor: Ivan-Krešimir Svetec (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2019-01-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Interdisciplinary Technical Sciences

Abstract

TRIM su mobilni elementi koji spadaju u skupinu neautonomnih retrotranspozona i do sada su dokazani u iznimno malom broju animalnih genoma. Školjkaši su skupina organizama još uvijek slabo okarakterizirana na genomskoj razini, no u najnovije vrijeme postali su predmetom istraţivanja jer je prepoznata njihova gospodarska i ekološka vaţnost. U sklopu ovog rada provedena su istraţivanja u svrhu detekcije i karakterizacije mobilnih elemenata TRIM u vrstama školjkaša Cerastoderma edule i Glycymeris glycymeris te njihova usporedba s elementima iz drugih vrsta školjkaša koji sadrţe ove retroelemente. Postojanje elemenata dokazano je analizom otisaka kolonija, odnosno bioinformatičkom analizom sekvenciranih uzoraka. Dobiven je postotak sličnosti iznad 90% unutar vrste, odnosno 75% izmeĎu ove dvije vrste. U drugom djelu ovog rada po prvi put je odreĎivan udio TRIM elemenata u genomu za svih 11 vrsta korištenih u ovom radu te je kapljičnom analizom dobiveno da kod svih vrsta iznosi <1% genoma.

Abstract (english)

TRIM mobile elements belong to the group of non-autonomous retrotransposons whose presence has been observed in very few animal genomes. Bivalves are a group of organisms still poorly explored at the genome level that have recently become the research object of interest due to their economic and ecological importance. Within this work, detection and characterization of TRIM mobile elements in the bivalve species Cerastoderma edule and Glycymeris glycymeris was carried out, followed by the comparison with elements from other bivalve species containing these retroelements. The existence of elements has been proved with the colony lift, actually with bioinformatics analysis of sequenced samples. There is a percentage of similarity above 90% within the species, or 75% between these two species. In the second part of this paper, for the first time, a determined fraction of the TRIM elements in the genome for all 11 species used in this paper was obtained with dot blot analysis and it was obtained that in all species is <1% of the genome.

• Keywords: TRIM mobilni elementi; školjkaši; retrotranspozoni
• Keywords (english): TRIM mobile elements; bivalves; retrotransposons
• Language: croatian
• URN:NBN: urn:nbn:hr:159:711303
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-12-06 13:41:06