Učinak proteinskog hidrolizata iz uljne pogače sjemenki konoplje na rast i produktivnost CHO DP-12 stanica

master's thesis

• Title (english): Effect of hemp seed meal protein hydrolysate on growth and productivity of CHO DP-12 cells
• Author: Karla Bojanić
• Mentor: Igor Slivac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2019-01-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Nusproizvodi prehrambene industrije poput uljne pogače konoplje, koja zaostaje nakon ekstrakcije ulja zbog izbalansiranog sastava aminokiselina predstavlja potencijalni supstrat u tehnologiji životinjskih stanica. U ovom radu ispitan je utjecaj medija s različitim udjelima FBS i proteinskog hidrolizata iz uljne pogače sjemenki konoplje dobivenog pomoću proteolitičkih enzima Alcalase i Protamex na rast i produktivnost CHO DP-12 stanica, proizvođača rekombinantnog humanog anti-IL8 te mogućnost zamjene dijela seruma hidrolizatom. Istraživanje je pokazalo da medij s 9% FBS i 1% hidrolizata dobivenog pomoću enzima Alcalase, te medij s 5-9% FBS i 1-5% hidrolizata dobivenog pomoću enzima Protamex predstavljaju pozitivan učinak na rast i produktivnost, dok u mediju s 1% FBS i 9% hidrolizata dolazi do inhibicije rasta. Pokazano je da hidrolizat ne može biti potpuna zamjena za serum, ali da u određenoj mjeri doprinosi povećanju rasta i produktivnosti.

Abstract (english)

By-products of food industry such as hemp seed meal, which remains after oil extraction because of amino-acid composition can potentially be used in animal cell technology. Therefore, in this study, the effect of medium containing different amount of FBS and hemp seed meal protein hydrolysate obtained using two proteolytic enzymes Alcalase and Protamex on growth and productivity of human recombinant anti-IL8 producing CHO DP-12 cells, was investigated as well as eventually substitution FBS by hydrolysate. The results showed that the medium containing 9% FBS and 1% hydrolysates obtained by Alcalase, and medium containing 5-9% FBS and 1-5% hydrolysates obtained by Protamex has positive effect on growth and productivity of CHO DP-12 cells, while medium containing 1% FBS and 9% hydrolysates has inhibitory effect. Also, it has been proven that this hydrolysate cannot be used as a substitute for serum, but might be a supplementation to achieve enhanced growth and productivity.

• Keywords: uljna pogača sjemenki konoplje; proteinski hidrolizat; monoklonsko protutijelo; CHO DP-12 stanična linija
• Keywords (english): hemp seed meal; protein hydrolysate; monoclonal antibody; CHO DP-12 cell line
• Language: croatian
• URN:NBN: urn:nbn:hr:159:463255
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-12-10 13:19:31