Selekcija kvasaca iz rodova Scheffersomyces, Candida i Spathaspora za industrijsku proizvodnju biokemikalija

master's thesis

• Title (english): Screening of yeast from genus Scheffersomyces, Candida and Spathaspora for industrial production of biochemicals
• Author: Anamarija Lončar
• Mentor: Antonija Trontel (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2019-10-23, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu provedena je selekcija kvasaca iz roda Scheffersomyces (Scheffersomyces insectosa JCM 9842, Scheffersomyces segobiensis JCM 7659), Candida (Candida lyxosophila JCM 7532, Candida succiphila JCM 9442 i Candida queiroziae CBS 11853) i Spathaspora (Spathaspora arborariae CBS 11463, Spathaspora passalidarum CBS 10155). Kvasci su uzgajani u YP podlogama u kojima su kao glavni izvor ugljika korišteni celobioza, glukoza, galaktoza, manoza, ksiloza i arabinoza. Nakon uzgoja određeni su glavni produkti metabolizma ovih kvasaca u aerobnim i anaerobnim uvjetima, a određen je i inhibitorni učinak različitih koncentracija mliječne kiseline na rast i aktivnost istraživanih kvasaca. Na temelju mogućnosti rasta na ugljikohidratima prisutnim u lignoceluloznim sirovinama i prema glavnim produktima metabolizma te otpornosti ovih kvasaca na visoke koncentracije mliječne kiseline odabrani su kvasci za daljnja istraživanja. Kvasci iz roda Spathaspora odabrani su zbog proizvodnje arabitola – biokemikalije koja se koristi kao zaslađivač. Proces proizvodnje arabitola s kvascima Spathaspora arborariae CBS 11463 i Spathaspora passalidarum CBS 10155 proveden je u YP podlozi s arabinozom u aerobnim uvjetima pri 28°C. Navedeni kvasci proizveli su arabitol s prinosom od 3,59 g L-1 i 3,25 g L-1 uz koeficijent konverzije od 0,63 i 0,46 g g-1. Kvasac Spathaspora arborariae CBS 11463 postiže oko 10 % veći prinos arabitola od kvasca Spathaspora passalidarum CBS 10155.

Abstract (english)

A selection of yeast from genus Scheffersomyces (Scheffersomyces insectosa JCM 9842, Scheffersomyces segobiensis JCM 7659), Candida (Candida lyxosophila JCM 7532, Candida succiphila JCM 9442 and Candida queiroziae CBS 11853) and Spathaspora (Spathaspora arborariae CBS 11463, Spathaspora passalidarum CBS 10155) was performed. Yeast were cultivated in YP medium with cellobiose, glucose, galactose, mannose, xylose and arabinose as main carbon sources. Main metabolic products were determined after cultivation in aerobic and anaerobic conditions, as well as inhibitory effect of lactic acid on growth and activity of tested yeast. Selection of yeasts for further experiments was made based on growth on the carbohydrates found in lignocelullose biomass, main metabolic products and tolerance to high concentration of lactic acid. Yeast from genus Spathaspora were selected because of their ability to produce a sweeterner arabitol. Production of arabitol with Spathaspora arborariae CBS 11463 and Spathaspora passalidarum CBS 10155 was performed in YP medium with arabinose in aerobic conditions at 28ºC. Spathaspora arborariae CBS 11463 and Spathaspora passalidarum CBS 10155 produced arabitol with yields of 3,59 g L-1 and 3,25 g L-1 and conversion of 0,63 i 0,46 g g-1. Spathaspora arborariae CBS 11463 achieved around 10 % higher arabitol yield than Spathaspora passalidarum CBS 10155.

• Keywords: proizvodnja biokemikalija; ne-Saccharomyces kvasci; Candida; Schefferosmyces; Spathaspora; arabitol; lignoceluloza
• Keywords (english): production of biochemicals; non-Saccharomyces yeast; Candida; Scheffersomyces; Spathaspora; arabitol; lignocelullose
• Language: croatian
• URN:NBN: urn:nbn:hr:159:814528
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-12-11 13:24:56