Biološka evaluacija sintetiziranih metoksiflavona

master's thesis

• Title (english): Biological evaluation of synthesized methoxyflavones
• Author: Martina Rajn
• Mentor: Kristina Radošević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2019-09-23, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Flavonoidi su raznovrsna skupina fitonutrijenata, bioaktivni polifenoli prisutni u voću, povrću i ljekovitim biljkama. Povezani su sa širokim spektrom učinaka u ljudskom tijelu koji povoljno utječu na zdravlje, a ponajviše se istražuje njihov antitumorski potencijal. S obzirom na malu apsorpciju prehrambenih flavonoida sintetizirani su metabolički stabilniji derivati, metoksiflavoni. U ovom radu ispitana je in vitro biološka aktivnost sedam različitih metoksiflavona na humanim tumorskim staničnim linijama (HeLa i MCF-7) te je određen mehanizam njihovog citotoksičnog djelovanja protočnom citometrijom i fluorescentnom mikroskopijom. Svih sedam metoksiflavona su pokazali inhibitorni učinak na staničnu liniju HeLa, te njih šest na MCF-7 pri čemu metoksiflavon 7 ima najveći inhibitorni učinak na proliferaciju stanica (IC50=57,323 μM). Također, praćenjem vijabilnosti stanica fluorescentnom mikroskopijom i određivanjem tipa stanične smrti u uzorcima tretiranim ispitivanim spojevima primjenom protočne citometrije pokazano je da metoksiflavon 7 ima najveći proapoptotički učinak. Dobiveni rezultati ukazuju na potencijal antitumorskog djelovanja ispitivanih metoksiflavona.

Abstract (english)

Flavonoids are a diverse group of phytonutrients, bioactive polyphenols present in fruits, vegetables and medicinal plants. They are associated with a wide spectrum of effects in human body that have a beneficial effect on health, and are mostly explored because of their anticancer potential. Given the small absorption of dietary flavonoids, metabolically more stable derivatives, methoxyflavones are synthesized. In this research, in vitro biological activity of seven different methoxyflavones on human tumor cell lines (HeLa and MCF-7) and a mechanism for their cytotoxic activity using flow cytometry and fluorescence microscopy were determined. All tested methoxyflavones showed inhibitory effect on the HeLa cell line, and six of them on MCF-7 where methoxyflavone 7 had the greatest inhibitory effect on cell lines proliferation (IC50= 57,323 μM). The results have also shown that methoxyflavone 7 had the greatest proapoptotic effect. The obtained results indicate the potential antitumor effect of the tested methoxyflavones.

• Keywords: antitumorsko djelovanje; flavonoidi; in vitro biološka aktivnost; metoksiflavoni; stanične linije
• Keywords (english): antitumor activity; flavonoids; in vitro biological activity; methoxyflavones; cell lines
• Language: croatian
• URN:NBN: urn:nbn:hr:159:162633
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-01-28 09:17:18