Optimizacija uvjeta fototrofnog uzgoja i karakterizacija dijatomeje izolirane iz Jadranskog mora

master's thesis

• Title (english): Optimization of phototrophic cultivation conditions and characterization of diatom isolated from the Adriatic sea
• Author: Antonija Brajković
• Mentor: Mirela Ivančić - Šantek (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2019-09-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Dijatomeje su prepoznate kao izvrsna sirovina za proizvodnju obnovljivih biogoriva, prehrambenih, farmaceutskih i nutraceutskih proizvoda. U ovom istraživanju optimiran je sastav podloge za fototrofni uzgoj dijatomeje izolirane iz Jadranskog mora. Najveća specifična brzina rasta od 0,371 dan -1 i koncentracije biomase od 0,47 g L-1 postignuta je na deset puta koncentriranoj f/2 podlozi. Proveden je polukontinuirani uzgoj dijatomeje kako bi se povećala gustoća fototrofne kulture. Maksimalna produktivnost biomase od 0,061 g L-1 d-1 i produktivnost biosinteze lipida od 14,64 mg L-1 d-1 postignuta je treći dan nakon prve prihrane. Najveća produktivnost biosinteze eikosapentaenoične kiseline od 3,28 mg L-1 dan-1 postignuta je u četvrtom danu uzgoja. Biomasa je stanica sadržavala klorofile i karotenoide, od kojih je najzastupljeniji bio fukoksantin. Metanolski i heksanski ekstrakti dijatomeje pokazali su snažan antibakterijski učinak na rast Staphylococcus aureus i Pseudomonas aeruroginosa.

Abstract (english)

Diatoms are being recognised as an excellent source for production of renewable biofuels, food, pharmaceutical and nutraceutical products. In the present research, composition of the growth media for phototrophic growth of diatom isolated from Adriatic Sea was optimized. The highest specific rate and biomass concentration of 0,371 day -1 and 0,47 g L-1, respectively, was achieved in ten times concentrated f/2 media. In order to increase cell density of phototrophic culture, repeated batch cultivation of diatom was conduct. The maximum biomass and lipid productivity of 0,061 g L-1 d-1 and 14,64 mg L-1 d-1, respectively, was reached third day after the first culture feed. The highest productivity of eicosapentaenoic acid of 3,28 mg L-1 dan-1 was obtained fourth day of cultivation. Cell biomass contained chlorophylls and carotenoids, with fucoxanthin as the most abundant pigment. Methanol and hexane extracts of diatoms showed strong antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa.

• Keywords: dijatomeje; lipidi; nutrijenti; antimikrobno djelovanje
• Keywords (english): diatoms; lipids; nutrients; antimicrobial activity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:879237
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-02-13 14:34:43