Učinci kurkumina na citotoksičnost induciranu polikloriranim bifenilima

master's thesis

• Title (english): Effects of curcumin on cytotoxicity caused by polychlorinated biphenyls
• Author: Dorotea Palić
• Mentor: Ivana Kmetič (mentor)
• Committee member: Teuta Murati (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2020-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

U ovom radu ispitan je protektivni učinak različitih koncentracija kurkumina (1 – 20 μM) pri citotoksičnom djelovanju 75 μM PCB-a 77 ili 75 μM PCB-a 153 na CHO (Chinese Hamster Ovary) – K1 stanicama metodama Trypan Blue, Neutral Red i Kenacid Blue. Utvrđeno je statistički značajno smanjenje vijabilnosti stanica u odnosu na kontrolne uzorke pri tretmanu CHO-K1 stanica s PCB-em 77 te PCB-em 153 metodama Trypan Blue i Kenacid Blue. Niti jedna od primijenjenih koncentracija kurkumina (1 - 20 μM) kojima su predinkubirane stanice nije potvrdila početnu hipotezu u o njegovom protektivnom učinku na citotoksičnost induciranu PCB 77. Toksični učinci dioksinu sličnog PCB kongenera u ovoj studiji nisu umanjeni tretmanom različitim koncentracijama kurkumina, čak štoviše, visoke koncentracije kurkumina (10 i 20 μM) dovele su do izraženijeg-sinergističkog učinka na smanjenje vijabilnosti stanica. Pri citotoksičnom djelovanju PCB-a 153, kurkumin je u pojedinim slučajevima (niže doze) pokazao blagi protektivni učinak na proliferaciju i vijabilnost CHO-K1 stanica.

Abstract (english)

In this study, the protective effect of different curcumin concentrations (1 – 20 μM) on cytotoxic activity of 75 μM PCB 77 or 75 μM PCB 153 on CHO (Chinese Hamster Ovary) – K1 cells was determined by Trypan Blue, Neutral Red and Kenacid Blue methods. Statistically significant reduction in cell viability was observed after treatment of CHO-K1 cells with PCB 77 or PCB 153 in comparison to the control samples determined with Trypan Blue and Kenacid Blue methods. The initial hypothesis based on curcumin protective effect on cytotoxicity induced by PCB 77 has not been confirmed. Toxic effects of dioxin like PCB congener in this study were not reduced by the action of the curcumin, on the contrary – high curcumin concentrations (10 and 20 μM) resulted in more pronounced effects on the reduction of cell viability. Low doses of curcumin showed a mild protective effect on PCB 153 induced reduction in proliferation and viability of CHO-K1 cells.

• Keywords: kurkumin; PCB 77; PCB 153; citotoksičnost; CHO-K1 stanična linija
• Keywords (english): curcumin; PCB 77; PCB 153; cytotoxicity; CHO-K1 cell line
• Language: croatian
• URN:NBN: urn:nbn:hr:159:531371
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-07-17 09:29:43