Uloga proteina uključenih u adheziju u otpornosti stanica karcinoma prostate DU145 na ionizirajuće zračenje

master's thesis

• Title (english): The role of adhesion proteins in the resistance of prostate carcinoma cell line DU145 to ionizing radiation
• Author: Marija Tomić
• Mentor: Ambriović Ristov Andreja (mentor); MBZ: 173311
• Committee member: Marina Cvjetko Bubalo (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2020-07-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Karcinom prostate jedan je od najčešćih zloćudnih tumora u muškaraca, a uspješno se liječi radioterapijom, odnosno zračenjem. Razvitak otpornosti stanica karcinoma na ionizirajuće zračenje ograničava uspjeh terapije, a jedan od mogućih mehanizama pojave ove otpornosti posredovan je promjenama u adheziji stanice posredovanoj integrinima. Cilj ovog rada bio je uspostaviti metodu za usporedbu kompleksa adhezije integrina stanične linije karcinoma prostate DU145 i sublinije otporne na zračenje DU145 RR. Izolirani kompleksi adhezije integrina analizirani su metodom western blot, a zamijećena je povećana ekspresija talina i α-aktinina u staničnoj liniji DU145 RR u odnosu na roditeljsku staničnu liniju DU145. Ovi rezultati upućuju na promjene u sastavu kompleksa adhezije integrina koji bi mogli biti povezani s otpornošću na radioterapiju. Na temelju rezultata ovog rada pripremit će se uzorci kompleksa adhezije integrina korištenog staničnog modela i analizirati spektrometrijom masa i moguće identificirati proteini promijenjeni u otpornoj staničnoj liniji koji bi mogli biti potencijalne mete za povećanje osjetljivosti stanica na radioterapiju.

Abstract (english)

Prostate cancer is one of the most common malignant tumors in men and it is successfully treated with radiotherapy. The development of radioresistance of cancer cells limits the therapy's success and one of the possible mechanisms of this resistance is mediated by changes in integrin-mediated cell adhesion. The aim of this study was to establish a method for comparing the integrin adhesion complex of the prostate cancer cell line DU145 and the radioresistant subline DU145 RR. Isolated integrin adhesion complexes were analyzed by the western blot method and an increased expression of talin and α-actinin was observed in the DU145 RR cell line relative to the parental DU145 cell line. These results suggest changes in the composition of integrin adhesion complexes that could be associated with radioresistance. Based on the results of this work, samples of integrin adhesion complexes of this cell model will be prepared and analyzed by mass spectrometry and it will be possible to identify proteins altered in the resistant cell line that could be potential targets for increasing cell susceptibility to radiotherapy.

• Keywords: karcinom prostate; otpornost na radioterapiju; adhezija stanica; adhesom; integrin
• Keywords (english): prostate cancer; radioresistance; cell adhesion; adhesome; integrin
• Language: croatian
• URN:NBN: urn:nbn:hr:159:012363
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-07-17 12:45:21