Utjecaj koncentracije izvora ugljika na kinetiku rasta ljubičaste nesumporne bakterije Rhodovulum adriaticum

undergraduate thesis

• Title (english): Effects of carbon source concentration on growth kinetics of the purple non-sulfur bacteria Rhodovulum adriaticum
• Author: Stella Brozović
• Mentor: Mario Novak (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2020-09-01, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Ljubičaste nesumporne bakterije predmet su istraživanja već godinama radi potencijalne primjene u biotehnološkoj proizvodnji različitih komercijalno vrijednih proizvoda kao što su biokemikalije, biopolimeri, vodik kao biogorivo, razni pigmenti i drugi spojevi. Da bi proces proizvodnje bio ekološki i ekonomski održiv potreban je obnovljiv i jeftin izvor sirovina, npr. lignoceluloza. Cilj ovog rada bilo je ispitivanje kinetike rasta ljubičaste nesumporne bakterije Rhodovulum adriaticum DSM 2781 na hranjivim podlogama s različitim koncentracijama izvora ugljika - glukoze i/ili ksiloze, koje čine većinski udio ugljikohidrata u hidroliziranim lignoceluloznim sirovinama. Tijekom uzgoja bakterije na podlozi s početnom koncentracijom glukoze od 1 g/L, zabilježen je najviši prinos biomase (1,839 g/L) te najveća produktivnost (0,019 g/(Lh)). Najniži prinos biomase (1,176 g/L) te najniža produktivnost (0,012 g/(Lh)) uočeni su prilikom uzgoja na hranjivoj podlozi s 10 g/L glukoze i 10 g/L ksiloze.

Abstract (english)

Purple non-sulfur bacteria have been the subject of research for years because of their potential use in the biotechnological production of various commercially valuable products such as biochemicals, biopolymers, hydrogen as a biofuel, pigments and other compounds. In order to achieve ecologically and economically sustainable production process, a renewable and cheap source of raw materials, e.g. lignocellulose, is required. The aim of this study was to research the growth kinetics of the purple non-sulfur bacterium Rhodovulum adriaticum DSM 2781 on different concentrations of carbon source – glucose and/or xylose, which make up the majority of sugars in hydrolysed lignocellulosic raw materials. The highest biomass yield (1,839 g/L) and the highest productivity (0,019 g/(Lh)) were achieved during the cultivation in the medium with 1 g/L glucose. While the lowest biomass yield (1,176 g/L) and the lowest productivity (0,012 g/(Lh)) were recorded during the growth with 10 g/L glucose and 10 g/L xylose.

• Keywords: izvor ugljika; lignocelulozne sirovine; ljubičaste nesumporne bakterije; Rhodovulum adriaticum
• Keywords (english): carbon source; lignocellulosic raw materials; purple non-sulfur bacteria; Rhodovulum adriaticum
• Language: croatian
• URN:NBN: urn:nbn:hr:159:257439
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-10-14 12:20:47