Primjena bakterijske nanoceluloze (BNC) u biotehnologiji i biomedicini

undergraduate thesis

• Title (english): Bacterial nanocellulose (BNC) application in biotechnology and biomedicine
• Author: Magdalena Barišić
• Mentor: Sunčica Beluhan (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2020-09-01, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Bakterijska nanoceluloza (BNC) je izrazito tanka, nanofibrilarna sirovina koja posjeduje jedinstvenu kombinaciju svojstava kao što su visoka kristaliničnost (84%-89%) i stupanj polimerizacije, velika površina (visoki udjel fibrila s promjerom od 20 do 100 nm), visoka fleksibilnost i vlačna snaga (Youngov modul od 15 do 18 GPa), kao i kapacitet zadržavanja vode (100 puta veći od svoje mase). Zahvaljujući velikoj strukturnoj čistoći jer nema lignina, hemiceluloze i pektina, BNC se smatra ne citotoksičnom, ne genotoksičnom i visoko kompatibilnom sirovinom, koja privlači interese znanstvenika iz različitih područja istraživanja, a posebice biomedicine. U ovom radu su pregledno prikazani biotehnološki aspekti proizvodnje BNC (vrste bakterija, izvori ugljika i hranjive podloge) i različite in situ i ex situ metode, primijenjene za BNC modifikaciju, posebice biogeničnog pristupa za primjenu u regenerativnoj biomedicini.

Abstract (english)

Bacterial nanocellulose (BNC) is ultrafine, nanofibrillar material with an exclusive combination of properties such as high crystallinity (84%–89%) and polymerization degree, high surface area (high aspect ratio of fibers with diameter 20–100 nm), high flexibility and tensile strength (Young modulus of 15–18 GPa), and high water-holding capacity (over 100 times of its own mass). Due to high structural purity because of absence of lignin, hemicellulose and pectin, BNC is considered as a non-cytotoxic, non-genotoxic and highly biocompatible material, attracting interest of scientists in diverse areas with hallmarks in biomedicine. The presented work summarizes the biotechnological aspects of BNC production (bacterial strains, carbon sources and media) and versatile in situ and ex situ methods applied in BNC modification, especially towards bionic design for applications in regenerative biomedicine.

• Keywords: bakterijska nanoceluloza; fermentacija; BNC modifikacija; kompoziti
• Keywords (english): Bacterial nanocellulose; Fermentation; BNC modification; Composite materials
• Language: croatian
• URN:NBN: urn:nbn:hr:159:009010
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-10-15 11:13:19