Metode određivanja antioksidacijske aktivnosti

undergraduate thesis

• Title (english): Methods for determination of antioxidant activity
• Author: Stela Galušić
• Mentor: Kristina Radošević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2020-09-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Antioksidansi su spojevi koji pomažu u neutraliziranju slobodnih radikala pa stoga njihovo ispitivanje privlači sve veći interes. Oksidacija izazvana slobodnim radikalima može izazvati razgradnju stanične membrane, mutacije u DNK i razvoj raznih bolesti ljudskog organizma. Za određivanje antioksidacijske aktivnosti nekog spoja koriste se niz metoda koje su zasnovane na različitim principima mjerenja. Ovaj rad daje uvid u razne kemijske metode za određivanje antioksidacijske aktivnosti. Većina tih metoda ne uzima u obzir biološke parametre potrebne za procjenu utjecaja antioksidansa u in vivo uvjetima. Metodom, kod koje se koriste in vitro kulture stanica, ispituje se potencijal antioksidansa u staničnom okruženju te se uzima u obzir složenost biološkog sustava i na taj način je moguća bolja procjena utjecaja ispitivanih spojeva u in vivo uvjetima.

Abstract (english)

Antioxidants are compounds that help neutralize free radicals and therefore there is an increasing interest for their determination. Oxidation caused by free radicals can cause the breakdown of the cell membrane, mutations in DNA and the development of various diseases of the human body. Various methods based on different reaction principles are used to determine the antioxidant activity of a compound. This paper provides insight into various chemical methods for the determination of antioxidant activity. Most of those methods do not take into account the biological parameters which are important for the estimation of antioxidant’s effect in vivo. The method based on in vitro cell culture examines the potential of antioxidants in the cellular environment and takes into account the complexity of the biological system. Therefore, such a method provides better activity assessment of the tested compounds related to the in vivo conditions.

• Keywords: antioksidansi; slobodni radikal; antioksidacijska aktivnost; metode
• Keywords (english): antioxidants; free radicals; antioxidant activity; methods
• Language: croatian
• URN:NBN: urn:nbn:hr:159:477579
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-10-27 08:00:41