Klonogena analiza nesteroidnih protuupalnih lijekova

undergraduate thesis

• Title (english): Clonogenic analysis of non-steroidal anti-inflammatory drugs
• Author: Lara Butumović
• Mentor: Kristina Radošević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2020-09-01, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Nesteroidni protuupalni lijekovi (NSAID, eng. non-steroidal anti-inflammatory drugs) u posljednje se vrijeme sve više istražuju zbog niza farmakoloških djelovanja, posebno kao potencijalni antikancerogeni lijekovi. Sposobni su inhibicijom enzima ciklooksigenaze umanjiti ili potpuno zaustaviti proliferaciju stanica, koja može dovesti do razvoja karcinoma. No, njihov mehanizam djelovanja još nije potpuno objašnjen zbog čega je potrebno provoditi dodatna ispitivanja vezana uz sigurnost, učinkovitost i toksičnost, ali i zbog pojave nuspojava uzrokovanih primjenom ovih lijekova. U ovom radu ispitivan je učinak pet različitih nesteroidnih protuupalnih lijekova (ibuprofen, vedaprofen, fluniksin, diklofenak i tolfenamatna kiselina) koristeći se in vitro metodom i kulturom stanica. Određivana je vijabilnost HeLa i HaCaT stanica nakon tretmana navedenim nesteroidnim protuupalnim lijekovima primjenom MTS kolorimetrijske metode i antikancerogena sposobnost NSAID-a primjenom klonogenog testa. Iz dobivenih rezultata može se zaključiti kako navedeni lijekovi posjeduju antiproliferatni učinak na tumorsku staničnu liniju, ali imaju utjecaja i na normalnu staničnu liniju, zbog čega su potrebna daljna istraživanja u tu svrhu. Inhibitorni učinak ovisan je o koncentraciji ispitivanog lijeka.

Abstract (english)

Lately, non-steroidal anti-inflammatory drugs (NSAID) have been more researched due to their pharmacological activity, especially it's potential as anticancer drugs. NSAIDs are capable of inhibiting the cyclooxygenase enzyme, by which they can completely stop the cell proliferation. Cell proliferation is the key process that contributes to development of tumor growth. But, their mechanism of action is not completely explained which raises the need to implement additional safety, efficiency and toxicological researches because of side effects caused by these drugs. In this work, five different non-steroidal anti-inflammatory drugs (ibuprofen, vedaprofen, flunixine, diclofenac and tolfenamatic acid) were tested using the in vitro method and cell culture. The viability of HeLa and HaCaT cells treated by selected NSAIDs was determined by the colorimetric MTS method and the anticancer ability using a clonogenic test. Obtained results indicate that tested drugs possess antiproliferative effect on the tumor cell line, as well as on the normal cell line. Observed inhibitory effect is dose dependent.

• Keywords: citotoksičnost; HaCaT stanična linija; HeLa stanična linija; klonogena analiza; nesteroidni protuupalni lijekovi
• Keywords (english): cytotoxicity; clonogenic assay; HaCaT cell line; HeLa cell line; nonsteroidal antiinflammatory drugs
• Language: croatian
• URN:NBN: urn:nbn:hr:159:090245
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-11-06 11:23:51