Bakteriocinska aktivnost bakterija mliječne kiseline izoliranih iz majčinog mlijeka

undergraduate thesis

• Title (english): Bacteriocin activity of lactic acid bacteria isolated from breast milk
• Author: Maja Horvat
• Mentor: Blaženka Kos (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2020-07-01, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Mnogi sojevi bakterija mliječne kiseline proizvode antimikrobne metabolite kao što su organske kiseline,ali i antimikrobni peptidi koji se nazivaju bakteriocini s potencijalom primjene u terapijske svrhe i kao biokonzervansi. Stoga je cilj ovog završnog rada bio metodičkim pristupom provesti izolaciju bakterija mliječne kiseline iz majčinog mlijeka i utvrditi njihovu bakteriocidnu aktivnost. Sojevi koji pokazuju bakteriocidno djelovanje detektirani su pomoću PCR reakcije sa specifičnim početnicama, a prisutnost bakteriocidnih proteina potvrđena je agaroznom gel elektroforezom amplifikacijskih produkata dobivenih PCR metodom. Pažljivo odabrani sojevi, koji pokazuju bakteriocidno djelovanje, imaju potencijalno antimikrobno djelovanje protiv humanih patogena, što doprinosi ograničavanju upotrebe antibiotika, a bakteriocini koje proizvode imaju potencijal primjene kao biokonzervansi u svrhu zaštite hrane.

Abstract (english)

Many strains of lactic acid bacteria produce antimicrobial metabolites such as organic acids, but also antimicrobial peptides called bacteriocins with the potential for use for therapeutic purposes and as biopreservatives. Therefore, the aim of this final work was to use a methodical approach to isolate lactic acid bacteria from breast milk and to determine their bactericidal activity. Strains showing bactericidal activity were detected by PCR reaction with specific primers, and the presence of bactericidal proteins was confirmed by agarose gel electrophoresis of amplification products obtained by PCR method. Carefully selected strains, which show bactericidal activity, have potential antimicrobial activity against human pathogens, which contributes to limiting the use of antibiotics, and the bacteriocins they produce have the potential to be used as biopreservatives for food protection purposes.

• Keywords: antibakterijska aktivnost; bakterije mliječne kiseljine; bakteriocini
• Keywords (english): antibacterial activity; lactic acid bacteria; bacteriocins
• Language: croatian
• URN:NBN: urn:nbn:hr:159:621020
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-11-16 10:17:40