Proizvodnja etanola s pomoću kvasaca iz roda Spathaspora na hidrolizatima dobivenim kiselinskom predobradom izluženih rezanaca šećerne repe

master's thesis

• Title (english): Ethanol production by yeasts from genus Spathaspora on hydrolisates obtained by acid pretreatment of leached sugar beet chips
• Author: Lucija Perković
• Mentor: Mario Novak (mentor)
• Committee member: Antonija Trontel (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2020-12-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Kvasci iz roda Spathaspora, uz heksoze (glukozu, galaktozu, manozu) mogu rasti i na pentozama te se iz tog razloga sve više istražuju u bioprocesima proizvodnje bioetanola i vrijednih biokemikalija kao što su šećerni alkoholi na lignoceluloznim sirovinama. U ovom radu provedena je kiselinska predobrada izluženih rezanaca šećerne repe u rasponu temperatura od 160 °C do 200 °C i vremenima zadržavanja od 1 do 10 minuta. Maksimalni udio ukupnih ugljikohidrata u hidrolizatu nakon predobrade određen je pri temperaturi od 200 °C i vremenu zadržavanja od 1 minute. Dobiveni hidrolizati s visokim udjelom ukupnih ugljikohidrata (160 °C/5 min, 180 °C/5 min, 180 °C/10 min, 200 °C/1 min i 200 °C/ 5 min) korišteni su za bioproces proizvodnje etanola i šećernih alkohola s pomoću kvasaca Spathaspora arborariae CBS 11463 i Spathaspora passalidarum CBS 10155 u mikroaerofilnim uvjetima. Navedeni kvasci u ispitivanim uvjetima proizvode etanol i šećerne alkohole u hidrolizatima dobivenim nakon predtretmana pri 160 °C/5 min i 180 °C/5 min. Tijekom uzgoja na hidrolizatima dobivenim pri 180 °C/10 min, 200 °C/1 min i 200 °C/5 min nije zamijećen rast niti jednog kvasca.

Abstract (english)

Yeasts of the genus Spathaspora can grow on pentose sugars, in addition to hexose sugars such as glucose, galactose and mannose. Therefore, they are being increasingly investigated for bioprocess production of bioethanol and valuble biochemicals such as sugar alcohols, primarily from lignocellulosic raw materials. In this paper, leached sugar beet chips were treated with diluted acid in the temperature range from 160 °C to 200 °C and retention time from 1 to 10 minutes. Highest amount of total carbohydrates in the hydrolyzate was determined at a temperature of 200 °C and retention time of 1 minute. The rest of the obtained hydrolyzates with high amount of total carbohydrates (160 °C/5 min, 180 °C/5 min, 180 °C/10 min, 200 °C/1 min and 200 °C/5 min) were used to produce ethanol and sugar alcohols using yeasts Spathaspora arborariae CBS 11463 and Spathaspora passalidarum CBS 10155 under microaerophilic conditions. These yeasts were showing growth under specific conditions on hydrolyzates obtained after pretreatment method under 160 °C/5 min and 180 °C/5 min. As for hydrolyzates obtained at 180 °C/10 min, 200 °C/1 min and 200 °C/5 min, no yeast growth was detected there.

• Keywords: izluženi rezanci šećerne repe; ne-Saccharomyces kvasci; etanol; šećerni alkoholi
• Keywords (english): leached sugar beet chips; Non-Saccharomyces yeasts; ethanol; sugar alcohols
• Language: croatian
• URN:NBN: urn:nbn:hr:159:186284
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-01-13 11:46:19