Ispitivanje antioksidacijske, antimikrobne i citotoksične aktivnosti ekstrakta bijele imele (Viscum album L.)

master's thesis

• Title (english): Analysis of antioxidative, antimicrobial and cytotoxic activity of white mistletoe extract (Vsicum album L.)
• Author: Alisa Hasanbegović
• Mentor: Jasna Mrvčić (mentor)
• Committee member: Kristina Radošević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Fermentation and Yeast Technology); Zagreb
• Defense date and country: 2020-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Bijela imela (Viscum album L.) je višegodišnja, zimzelena, grmolika, poluparazitska biljka koja raste na različitim stablima. Od 1917. godine imelini pripravci se koriste u antitumorskim terapijama i danas se često koriste u komplementarnoj medicini u liječenju tumora. Glavne citotoksične komponente imele su lektini i viskotoksini. Istraživanja imele pokazala su njene brojne biološke učinke. Osim antitumorskih pokazuje i antimikrobne, antioksidacijske, antivirusne te imunomodulatorne aktivnosti. Cilj istraživanja bio je odrediti antioksidacijsku, antimikrobnu i citotoksičnu aktivnost ekstrakata bijele imele pripremljenih maceracijom u vodeno-etanolnoj bazi pri čemu su u obzir uzeti sljedeći parametri maceracije: masena koncentracija biljnog materijala 20, 40 i 80 g Lˉ¹ i volumni udio etanola u vodeno-etanolnoj bazi 25, 40, 55 i 70 %. S obzirom da eksperimenti za određivanje antimikrobne i citototoksične aktivnosti nisu provedeni zbog pandemije uzrokovane virusom SARS-CoV-2 u laboratoriju, u radu su prikazani dosadašnji rezultati objavljeni u literaturi.

Abstract (english)

White mistletoe (Viscum album L.) is a perennial, evergreen, shrubby, semi-parasitic plant that grows on various host trees. Since 1917, mistletoe preparations have been used in antitumor therapies and today are often used in complementary medicine in the treatment of tumors. The main cytotoxic components of mistletoe are lectins and viscotoxins. Mistletoe research has shown its numerous biological effects. In addition to anticancer, it also shows antimicrobial, antioxidant, antiviral and immunomodulatory activities. The aim of the study was to determine the antioxidant, antimicrobial and cytotoxic activity of white mistletoe extracts prepared by maceration of water-ethanol base, taking into account the following maceration parameters: mass concentration of plant material 20, 40 and 80 g Lˉ¹ and volume fraction of ethanol in water-ethanol bases 25, 40, 55 and 70 %. Since experiments to determine antimicrobial and cytotoxic activity in the laboratory have not been conducted due to a pandemic caused by the SARS-CoV-2 virus, this part of the paper provides an overview of the results published so far in the literature for the said experiments.

• Keywords: bijela imela; viskotoksini; lektini; maceracija
• Keywords (english): white mistletoe; viscotoxins; lectins; maceration
• Language: croatian
• URN:NBN: urn:nbn:hr:159:985476
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-01-20 13:40:27