Identifikacija sojeva i analiza prisutnosti S-proteina bakterija mliječne kiseline izoliranih iz majčinog mlijeka

master's thesis

• Title (english): Identification and analysis of the presence of S-layer proteins on the lactic acid bacteria isolated from mother's milk
• Author: Anđelina Bošković
• Mentor: Andreja Leboš Pavunc (mentor)
• Committee member: Jasna Novak (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2021-03-04, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Probiotici su živi mikroorganizmi, koji u dovoljnom broju, mogu povoljno utjecati na zdravstveno stanje potrošača. Bakterije mliječne kiseline, osobito one iz rodova Lactobacillus i Bifidobacterium, imaju velike predispozicije za probiotičko djelovanje. S-proteini dokazano poboljšavaju probiotički učinak stoga je cilj ovog rada bio ispitati posjeduju li na svojoj površini S-proteine bakterije izolirane iz majčinog mlijeka. Majčino mlijeko je izvor brojnih nutritivnih i biološki aktivnih tvari pa kao takvo predstavlja pogodno prirodno stanište bakterijama mliječne kiseline. Identifikacijom se ustvrdilo da su najzastupljeniji mikroorganizmi majčinog mlijeka bakterije iz roda Staphylococcus, ali i bakterije iz rodova Lactobacillus i Bifidobacterium su bile značajno zastupljene (24 %). Također je eksperimentalno dokazano da izolirani bakterijski sojevi ne posjeduju S-proteine na svojoj površini odnosno ne posjeduju gene koji bi kodirali za S-proteine.

Abstract (english)

Probiotics are living microorganisms, which in sufficient number can have a beneficial effect on the health of the consumer. Lactic acid bacteria, especially genera Lactobacillus and Bifidobacterium, have a big predisposition for probiotic action. S-proteins have been shown to improve probiotic effect so the aim of this study was to examine whether bacteria isolated from breast milk, possess S-proteins. Breast milk is a source of man nutrients and biologically active substances and as such, it is suitable natural habitat for lactic acid bacteria. The identification established that the most common microorganisms in breast milk are bacteria from the genus Staphylococcus but also bacteria from the genera Lactobacillus and Bifidobacterium were present in higher abundance (24 %). It has also been experimentally proven that isolated bacteria do not possess S-proteins on their surface respectively they do not possess genes that would encode S-proteins.

• Keywords: probiotici; bakterije mliječne kiseline; S-proteini; majčino mlijeko
• Keywords (english): probiotics; lactic acid bacteria; S-proteins; breast milk
• Language: croatian
• URN:NBN: urn:nbn:hr:159:509355
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-03-17 10:39:45