Genotoksični učinak terapijskih doza Δ9-tetrahidrokanabinola i irinotekana na humane stanične linije karcinoma crijeva i jetre

master's thesis

• Title (english): Genotoxic effect of therapeutic doses of Δ9-tetrahydrocannabinol and irinotecan on human colon and liver cancer cell lines
• Author: Ana - Marija Genc
• Mentor: Ksenija Durgo (mentor)
• Committee member: Draženka Komes (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2021-01-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Interdisciplinary Technical Sciences

Abstract

U većini zemalja svijeta, maligna su oboljenja po učestalosti drugi uzrok smrtnosti stanovništva. O samoj vrsti tumora i njegovoj uznapredovalosti ovisi način liječenja, koji najčešće zahtijeva multimodalni pristup. Irinotekan je jedan od najčešće primjenjivanih citostatika za liječenje metastatskog kolorektalnog karcinoma. Δ9-tetrahidrokanabinol (THC) je glavna aktivna komponenta konoplje čija se uporaba u medicinske svrhe aktivno razmatra posljednjih nekoliko desetljeća. Cilj ovog rada je istražiti citotoksičnost, genotoksičnost i prooksidacijski učinak različitih koncentracija irinotekana i THC-a. Kao test sustavi korištene su humana stanična linija hepatocelularnog karcinoma jetre (HepG2) i humana stanična linija adenokarcinoma epitela debelog crijeva (Caco-2) koje su bile izložene djelovanju irinotekana i THC-a tijekom 2, 4, 24 i 48 sati. Dokazano je da irinotekan i THC djeluju citotoksično ovisno o duljini tretmana stanica i primijenjenoj koncentraciji. Najveći prooksidacijski učinak zabilježen je na obje stanične linije nakon 4 sata, dok genotoksično djelovanje ponajviše uvjetuju više koncentracije irinotekana.

Abstract (english)

In most countries, cancer is the second leading cause of death. The type of tumor and the stage of the disease will depend on the exact treatment, which usually involves a combination of therapies. Irinotecan is the key anti-cancer drug for the treatment of metastatic colorectal cancer. Δ9-tetrahydrocannabinol (THC) is the main active compound in the Cannabis plant which has been increasingly used in medicine over the past decades. The aim of this work is to investigate the cytotoxicity, genotoxicity and pro-oxidative effects of different concentrations of irinotecan and THC. As a test system, the human hepatocellular carcinoma cell line (HepG2) and the human colon adenocarcinoma cell line (Caco-2) were exposed to irinotecan and THC during 2, 4, 24, and 48 hours. It has been shown that the cytotoxicity of irinotecan and THC depends on the duration of cell treatments and concentration used. The highest pro-oxidative effect was observed at 4 hours for both cell lines, while genotoxic activity was mostly caused by higher concentrations of irinotecan.

• Keywords: Δ9-tetrahidrokanabinol (THC); irinotekan; citotoksičnost; genotoksičnost; stanične linije HepG2 i Caco-2; in vitro testovi
• Keywords (english): Δ9-tetrahydrocannabinol (THC); irinotecan; cytotoxicity; genotoxicity; HepG2 and Caco-2 cell lines; in vitro assays
• Language: croatian
• URN:NBN: urn:nbn:hr:159:333785
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-03-17 12:28:06