Karakterizacija probiotičkih bakterija za primjenu u zaštiti dentooralnog zdravlja

undergraduate thesis

• Title (english): Characterization of probiotic bacteria for application in dentooral health protection
• Author: Marija Tankosić
• Mentor: Jadranka Frece (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2021-06-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Zbog loše oralne higijene u Republici Hrvatskoj se sve češće javljaju dentooralna oboljenja koja predstavljaju veliku opasnost za čovjekovo zdravlje. Skupocjenost i dugotrajnost samog liječenja potaknule su znanstvenike diljem svijeta na istraživanja i pronalazak novih metoda prevencija istih. Uz bolju oralnu higijenu, korištenjem fluora u raznim varijantama, zanimljivom se pokazala upotreba bakterija mliječne kiseline s probiotičkim djelovanjem. Cilj ovoga rada bilo je ispitati probiotički potencijal odabranih sojeva bakterija mliječne kiseline i vidjeti kakav će utjecaj imati na inhibiciju stvaranja biofilmova uzročnika dentooralnih tegoba:Streptococcus mutans, Streptococcus sanguinis, Streptococcus intermedius te Aggregatibacter actinomycetemcomitans. Obzirom da se radi o usnoj šupljini, u uvjetima simulirane sline određena je sposobnost preživljenja probiotika te autoagregacija i koagregacija probiotičkih bakterija Lactobacillus plantarum KO9 i Lactobacillus plantarum M1 s navedenim patogenima. Rezultati su pokazali visok stupanj preživljenja u uvjetima sline te značajnu sposobnost koagregacije i inhibicije biofilmova bakterija uzročnika dentooralnih oboljenja.

Abstract (english)

Due to poor oral hygiene in the Republic of Croatia, dentooral diseases are increasingly occurring, which pose a great danger to human health. The costliness and longevity of the treatment itself have encouraged scientists around the world to research and find new methods of prevention. In addition to better oral hygiene, the use of fluoride and the use of lactic acid bacteria with probiotic potential proved to be interesting. The aim of this study was to examine the probiotic potential of selected strains of lactic acid bacteria and see what effect they will have on the inhibition of biofilm production of dental problems causing:Streptococcus mutans, Streptococcus sanguinis, Streptococcus intermedius and Aggregatibacter actinomycetemcomitans. Since it is an oral cavity, the ability of probiotic basteria Lactobacillus plantarum KO9 and Lactobacillus plantarum M1to survive in simulated saliva and autoaggregate and coaggregatewith the mentioned pathogens was determined The results showed a high survival rate under saliva conditions and a significant ability to coaggregate and inhibit biofilms of bacteria causing dentoral diseases.

• Keywords: dentalni patogeni; dentooralna oboljenja; probiotici
• Keywords (english): probiotics; dental phatogen; dentoral disease
• Language: croatian
• URN:NBN: urn:nbn:hr:159:964237
• Study programme: Title: Food Technology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-07-14 09:59:27