Proizvodnja biokemikalije s pomoću plijesni Mucor indicus u različitim tipovima bioreaktora i obrada otpadne prevrele podloge nesumpornim ljubičastim bakterijama

undergraduate thesis

• Title (english): Biochemicals production with Mucor indicus in different bioreactors and treatment of the fermented media with purple non-sulfur bacteria
• Author: Mija Kaselj
• Mentor: Mario Novak (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2021-07-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Sve veće potrebe za ekološki i ekonmski održivim proizvodnjama dovele su do razvoja biorafinerija i iskorištavanja obnovljivih izvora energije. Biorafinerija je integrativni i višenamjenski koncept koji koristi lignoceluloznu biomasu za održivu proizvodnju, a uključuje selektivnu transformaciju različitih molekula biomase u biokemikalije, biopolimere, biogoriva i druge spojeve. Cilj ovog rada je ispitati utjecaj različitih tipova bioreaktora na rast plijesni Mucor indicus čija biomasa je bogata masnim kiselinama, dok je proizvod asimilacije šećera iz hranjive podloge ksilitol. Uzgojem plijesni Mucor indicus u biorekatoru s mješalom proizvelo se 6,634393 g/L ksilitola, 3,5414 g/L suhe tvari biomase koja je sadržavala 72,5 % nezasićenih masnih kiselina s najvećim udjelom cis-9 oktadekanoična kiseline u postotku od 50,5 %. Kad se plijesan Mucor indicus uzgajala u babotirajućem bioreaktoru dobilo se 0,750836 g/L ksilitola, 4,624 g/L biomase koja je sadržavala 82,4 % nezasićenih masnih kiselina s najvećim udjelom cis-9-oktadekanoične kiseline u postotku od 47,11 %. Kako bi se dodatno smanjila količina otpada, na iskorištenim podlogama iz uzgoja plijesni proveo se uzgoj fototrofnih ljubičastih nesumpornih bakterija u svrhu iscrpljivanja izvora ugljika i dušika iz navedenih podloga. Tijekom uzgoja ljubičastih nesumpornih bakterija pratio se utjecaj različitih izvora svjetlosti, na rast i sintezu bakterioklorofila a.

Abstract (english)

The growing need for both ecologically and economically sustainable production systems has brought to the development of biorefineries and the use of renewable raw materials. A biorefinery is an integrative and multipurpose concept that uses lignocellulosic biomass for sustainable production which includes a selective transformation of different biomass molecules into biochemicals, biopolymers, biofuel, and other components. The purpose of this thesis is to examine the influence of different bioreactor types on Mucor indicus growth, whose biomass is rich with fatty acids whereas the product of sugar assimilation is xylitol. Cultivating Mucor indicus in a stirred-tank bioreactor resulted in the production of 6,634393 g/L of xylitol and 3,5414 g/L of dry biomass that consisted of 72,5 % non-saturated fatty acids with cis-9 octadecanoic acid having the largest ratio of 50,5 %. When the Mucor indicus was cultivated in a sparged tank bioreactor, the amount of xylitol and biomass produced was 0,750836 g/L and 4,624 g/L respectively, whereas the dry biomass consisted of 82,4 % of non-saturated fatty acids with the largest ratio of cis-9-octadecanoic acid of 47,11 %. To reduce the total amount of waste from the cultivations and to use the carbon and nitrogen left-overs, the fermented broth was used for the cultivation of phototrophic purple non-sulfur bacteria, during which the influence of different light sources on the growth and bacteriochlorophyll a synthesis was examined.

• Keywords: lignocelulozne sirovine; Mucor indicus; biorafinerija; biokemikalije; ljubičaste nesumporne bakterije; Rhodovulum adriaticum DSM 2718; Rhodobacter azotoformans JCM 9340; Rhodovulum capsulatus JCM 21090; izvor svjetlosti
• Keywords (english): lignocellulosic raw materials; Mucor indicus; biorefinery; biochemicals; purple non-sulfur bacteria; Rhodovulum adriaticum DSM 2718; Rhodobacter azotoformans JCM 9340; Rhodovulum capsulatus JCM 21090; light source
• Language: croatian
• URN:NBN: urn:nbn:hr:159:859851
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-07-28 12:04:05