Priprava i spektroskopska analiza ferocenskih dipeptida izvedenih iz ferocen-1,1'-diamina i valina

undergraduate thesis

• Title (english): Preparation and spectroscopic analysis of ferrocene dipeptides derived from ferrocene-1,1'-diamine and valine
• Author: Klara Širić
• Mentor: Monika Kovačević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Organic Chemistry); Zagreb
• Defense date and country: 2021-09-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Sekundarna struktura proteina ima bitnu ulogu u konačnoj strukturi proteina, stabilnosti, presavijanju, biološkim procesima i u samoj funkciji proteina, te se stoga pokušava predvidjeti i usmjeriti, kako bi proteini postali konformacijski fleksibilniji, stabilniji i time terapeutski primjenjiviji. U ovom je radu opisana sinteza i konformacijska analiza biokonjugata Boc-D-Val-NH-Fn- COOMe (1) i Ac-D-Val-NH-Fn-COOMe (2) (Ac = acetil, Boc = tert-butoksikarbonil) koji su pripravljeni iz voluminozne aminokiseline valina i ferocenske aminokiseline. S ciljem utvrđivanja mogućeg steričkog utjecaja valinskog bočnog ogranaka na uspostavu sekundarne strukture rezultirajućih peptida, provedena je konformacijska analiza (IR-, NMR- i CD-spektroskopija).

Abstract (english)

The secondary structure of a protein plays an important role in the final structure of the protein, stability, folding, biological processes and in the function of the protein itself. Therefore, scientific researches are made to predict and direct proteins to be more conformationally flexible, stable and therapeutically applicable. This paper describes the synthesis and conformational analysis of the bioconjugates Boc-D-Val-NH-Fn-COOMe (1) and Ac-D-Val-NH-Fn-COOMe (2) (Ac = acetyl, Boc = tert-butoxycarbonyl) which are prepared from the voluminous amino acid valine and the ferrocene amino acid. In order to determine the possible steric influence of the valine backbone on the formation of the secondary structure of the resulting peptides, conformational analysis (IR, NMR and CD spectroscopy) was performed.

• Keywords: ferocen; konformacijska analiza; valin; peptidomimetici; vodikova veza
• Keywords (english): ferrocene; conformational analysis; valine; peptidomimetics; hydrogen bond
• Language: croatian
• URN:NBN: urn:nbn:hr:159:157021
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-09-24 09:59:46