Genotoksičnost procjednih voda s odlagališta otpada Mraclinska Dubrava, ljeto 2014.

master's thesis

• Title (english): Genotoxicity of landfill leachate from the waste Mraclinska Dubrava, summer 2014.
• Author: Petra Roko
• Mentor: Ksenija Durgo (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2014-12-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Porastom industrijalizacije i urbanizacije dolazi do sve većeg nakupljanja otpada, koji predstavlja prijetnju za okoliš i živi svijet. Odlaganjem otpada na odlagališna zemljišta, dolazi do kontaminacije tla, a time i stvaranja procjednih voda i onečišćenja podzemnih voda. U ovom radu je ispitivana genotoksičnost podzemnih i procjedne vode odlagališta otpada „Mraclinska Dubrava“. Određivana je citotoksičnost, prooksidativni učinak i lipidna peroksidacija na stanicama raka grkljana, HEp2 te mutageni učinak na sojevima bakterija Sallmonela typhimurium (TA 98 i TA 100). Procjedna voda pokazala je prooksidativni i citotoksični učinak u ovisnosti o koncentraciji, dok je podzemna voda uzorkovana kod samog pročiščivača izazivala lipidnu peroksidaciju. Podzemna voda uzorkovana ispod pročiščivača dovela je do indukcije slobodnih radikala koji nisu izazvali oštećenje lipida.

Abstract (english)

Increased industrialization and urbanization leads to the growing accumulation of waste, which poses a threat to the environment and wildlife. Consequently waste disposal to landfill soil becomes contaminated soil, and thus the creation of leachate and groundwater pollution, water for human consumption. In this study we examined the genotoxicity of groundwater and leachate landfill "Mraclinska Dubrava". Cytotoxicity, induction of free radicals and lipid peroxidation was determined on laryngeal cancer cell line, HEp2, while mutagenicity was tested on Sallmonela typhimurium (TA 98 i TA 100) bacterial strains. Leachate caused prooxidative and cytotoxic effect in dose dependent manner, while the ground water collected under water purifier facility caused lipid peroxidation. Ground water collected down bellow water purifier induced free radicals which did not damage cellular lipids.

• Keywords: procjedna voda; otpadna voda; genotoksičnost; „Mraclinska Dubrava“; Salmonella typhimurium; stanična linija karcinoma grkljana HEp2
• Keywords (english): leachate; wastewater; genotoxicity; „Mraclinska Dubrava“; Salmonella typhimurium; HEp2 laryngeal carcinoma cell line.
• Language: croatian
• URN:NBN: urn:nbn:hr:159:619762
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-27 08:18:27