Inhibicijski učinak propolisa na klijavost zoospora patogenih oomiceta Saprolegnia parasitica i Aphanom yces astaci

undergraduate thesis

• Title (english): Inhibitory effect of propolis on germination of zoospores of pathogenic oomycetes Saprolegnia parasitica and Aphanom yces astaci
• Author: Karla Vasari
• Mentor: Ana Bielen (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2021-09-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Patogene oomicete Saprolegnia parasitica i Aphanomyces astaci uzročnici su dominantnih bolesti u slatkovodnoj akvakulturi. Postojeće kemikalije za njihovo suzbijanje toksične su za domaćina, ljude i okoliš, te je potrebno razviti nove, ekološki prihvatljive i održive metode. Cilj ovog rada bio je istražiti antioomicetno djelovanje propolisa i njegovih glavnih komponenti. Korištena su dva pripravka propolisa: P1 - 200 mg mLˉ¹ i P2 - 250 mg mLˉ¹, s dodatkom ekstrakata kadulje i paprene metvice, te su oba inhibirala klijavost zoospora oomiceta S. parasitica i A. astaci s EC₅₀ vrijednostima od 15 do 24 μg mLˉ¹. Nadalje, primjena krizina, kao dominantne komponente propolisa, uzrokovala je inhibiciju klijavosti zoospora vrste S. parasitica, ali u koncentraciji višestruko višoj od one u pripravcima propolisa. Zaključno, po prvi je puta demonstrirana inhibicija klijavosti zoospora oomiceta propolisom, za koju se može pretpostaviti da je posljedica sinergije većeg broja bioaktivnih komponenti.

Abstract (english)

The pathogenic oomycetes Saprolegnia parasitica and Aphanomyces astaci are the causative agents of the most important diseases in freshwater aquaculture. Their control is based on agents that are toxic to the host, humans and the environment. Therefore, new, environmentally friendly and sustainable methods need to be developed. The aim of this study was to investigate the antioomycetic activity of propolis and its main components. Two propolis solutions were used: P1 (200 mg mLˉ¹) and P2 (250 mg mLˉ¹, with the addition of sage and peppermint extracts), both of which inhibited S. parasitica and A. astaci zoospore germination with EC₅₀ values of 15 - 24 μg mLˉ¹. In addition, chrysin, the dominant component of propolis, caused an inhibition of zoospore germination of S. parasitica, but at a higher concentration than in propolis solutions. In summary, the inhibition of zoospore germination of oomycetes by propolis, demonstrated for the first time, is probably due to the synergy of multiple bioactive components.

• Keywords: Aphanomyces; krizin; propolis; Saprolegnia; zoospore
• Keywords (english): Aphanomyces; chrysin; propolis; Saprolegnia; zoospore
• Language: croatian
• URN:NBN: urn:nbn:hr:159:239255
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-09-29 10:08:41