Istraživanje mehanizma djelovanja male kemijske molekule ciljanjem proteina LINGO-1 kao potencijalno sredstvo u borbi protiv glioblastoma

master's thesis

• Title (english): Study the mechanism of action of a small chemical molecule targeting LINGO-1 as potential anti-tumor agent in glioblastoma
• Author: Ana Novak
• Mentor: Vladimir Mrša (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Biochemistry); Zagreb
• Defense date and country: 2021-09-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Glioblastomas (GBM) are the most frequent and aggressive form of gliomas. Despite standard treatment with maximal surgical resection, followed by radiation and chemotherapy with temozolomide (TMZ), the prognosis of patients with glioblastomas remains very poor (Stupp et al., 2005). Median survival of patients is no longer than 15 months (Koshy et al., 2012). In general, the conventional treatments of GBM are very limited and deceiving because of the high resistance for the treatments. In this context, identification of other pharmacological targets regulating signaling pathways involved in the cell survival and proliferation could open new windows for GBM therapies. In this study, our aim is to explore the role of a protein LINGO-1 in physiopathology of GBM, which is a transmembrane protein belongs to the family LRIG (Leucine Rich and Immunoglobulin domain) and expressed in central and peripheral nervous system. Several arguments showed that LINGO-1 could be a very interesting target and is still waiting to be explored in GBM. In this project for internship, we want to explore the mechanism of action of one molecule targeting LINGO-1 to understand the signaling pathways involved in its anti-tumor activity in glioblastoma cells.

Abstract (croatian)

Glioblastom (GBM) je najčešći i najagresivniji oblik glioma tumora. Unatoč standardnom liječenju uključujući kiruršku sekciju, nakon čega slijede zračenje i kemoterapija temozolomidom (TMZ), prognoza pacijenata oboljelih od glioblastoma ostaje vrlo loša (Stupp i sur., 2005.). Prosječno preživljenje pacijenata nije duže od 15 mjeseci (Koshy i sur., 2012.). Općenito, konvencionalni tretmani GBM-a vrlo su ograničeni i neučinkoviti zbog velike otpornosti bolesti na postojeće tretmane. U tom kontekstu, identifikacija drugih farmakoloških ciljeva koji reguliraju signalne putove uključene u preživljavanje i proliferaciju stanica glioblastoma mogla bi otvoriti nove mogućnosti za liječenje i terapiju tog smrtonosnog tumora. U ovom radu cilj nam je istražiti ulogu proteina LINGO-1 u fiziopatologiji GBM-a. LINGO-1 je transmembranski protein koji pripada obitelji LRIG (domena bogata leucinom i sadrži imunoglobulinsku domenu) i izražen je u središnjem i perifernom živčanom sustavu. Nekoliko je argumenata pokazalo da bi LINGO-1 mogao biti vrlo zanimljiva meta i da još uvijek čeka na istraživanje u GBM-u. U ovom projektu u sklopu izrade diplomskog rada želimo istražiti mehanizam djelovanja jedne molekule koja cilja LINGO-1 kako bismo razumjeli signalne putove uključene u njezino protutumorsko djelovanje u stanicama glioblastoma.

• Keywords: gliomas; glioblastoma; LINGO-1; anti-tumor activity
• Keywords (english): LINGO-1; glioblastom; tumor; glioma
• Language: english
• URN:NBN: urn:nbn:hr:159:072050
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Closed access
• Created on: 2021-10-04 10:33:04