Ispitivanje učinka biološki aktivnih tvari iz kore cikle i kore banane na stanične linije i makromolekule

master's thesis

• Title (english): Effects of biologically active compounds from banana peel and red beetroot peel on cell lines and macromolecules
• Author: Anamaria Komljenović
• Mentor: Ksenija Durgo (mentor)
• Committee member: Višnja Gaurina-Srček (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2021-09-23, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Interdisciplinary Technical Sciences

Abstract

Prilikom prerade cikle i banane proizvodi se velika količina otpada koja se može iskoristiti u različite svrhe. Kora cikle i banane kao nusproizvod sadržava fitokemikalije koje imaju izraženo antioksidacijsko djelovanje koje ima ulogu u zaštiti od različitih kroničnih i kardiovaskularnih bolesti. Cilj rada je istražiti biološki učinak ekstrakta kore cikle i kore banane na stanične makromolekule te humane stanične linije karcinoma debelog crijeva (Caco-2), karcinoma usne šupljine (Cal27) i hepatocelularnog karcinoma jetre Hep G2 u koncentracijama za koje je realno očekivati da budu unesene u organizam tijekom 24 sata. Rezultati su pokazali da fitokemikalije iz kore cikle i kore banane djeluju protektivno na lipide, da izazivaju genotoksični učinak samo na staničnoj liniji jetre te da pri višim koncentracijama induciraju glutation. Istraživani uzorci nisu pokazali značajno antioksidativno djelovanje na modelnim molekulama DNA i proteina dok u određenim uvjetima imaju i prooksidativno djelovanje.

Abstract (english)

A large amount of waste is produced when red beetroot and bananas are processed. Their by-products can be used for various purposes and they contain phytochemicals that have antioxidative effect that has a role in protection of various chronic and cardiovasular diseases. The aim of this study is to determine biological effect of beetroot peel and banana peel extracts on cellular macromolecules, human colon cancer cell line Caco-2, human oral carcinoma cell line Cal27 and hepatocellular liver carcinoma cell line Hep G2 in concentrations that are expected to be consumed over a 24 hour period. The results showed that phytochemicals contained in the beetroot peel and banana peel have a protective effect on lipids while they do have genotoxic effect only on HepG2 cell line and they induce glutathione at higher concentrations. Used extracts did not show a significant antioxidative effect on DNA model and protein molecules, while under certain conditions they also have a prooxidative effect.

• Keywords: kora cikle; kora banane; biološki aktivne tvari; stanične linije; stanične makromolekule
• Keywords (english): red beetroot peel; banana peel; biologically active compounds; cell lines; macromolecules
• Language: croatian
• URN:NBN: urn:nbn:hr:159:116706
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-10-11 12:45:07