Probiotička karakterizacija bakterija mliječne kiseline izoliranih iz stolice dojenčeta

master's thesis

• Title (english): Probiotic characterization of lactic acid bacteria isolated from infant feces
• Author: Mia Vodjerek
• Mentor: Jadranka Frece (mentor)
• Committee member: Ksenija Markov (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2021-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Mikrobiološka raznolikost crijevne mikrobiote omogućava odvijanje ključnih metaboličkih procesa, ravnotežu imunosnog sustava, otpor patogenim mirkoorganizmima te mnoge druge korisne učinke. Ako je mikrobna ravnoteža narušena može doći do razvoja bolesti i mnogih zdravstvenih poremećaja. Način života, prehrana, stres, lijekovi i mnogi drugi čimbenici imaju direktan utjecaj na navedenu ravnotežu. Dokazano je da probiotički preparati mogu povoljno djelovati na narušenu crijevnu mikrobiotu,... More uspostavljajući ravnotežu, inhibirajući patogene mikroorganizme, potičući imunosni sustav te ispoljavajući mnoge druge korisne učinke. Korišteni probiotici moraju zadovoljiti niz kriterija kako bi se osiguralo željeno djelovanje na ciljanom mjestu u domaćinu i otklonili mogući negativni utjecaji. Kako bi se navedeno osiguralo, provodi se probiotička karakterizacija s ciljem isptivanja probiotičkih kandidata na niz kriterija te procjena njihove uspješnosti i konačno probiotičkog potencijala. Stolica dojenčeta smatra se dobrim izvorom potencijalnih probiotika jer su mnoga istraživanja pokazala kako je to mjesto kvalitetnih probiotičkih sojeva koji su dokazali svoje korisne učinke na zdravlje. U ovom radu, provedena je probiotička karakterizacija deset izolata bakterija mliječne kiseline iz stolice dojenčeta. Odabran je najbolji soj, Lactobacillus plantarum B12, koji je pokazao visoko preživljenje u simuliranim uvjetima gastrointestinalnog sustava, antimikrobnu aktivnost i koagregaciju s patogenima, antioksidativnu aktivnost i hidrofobnost stanične površine. L. plantarum B12 zadovoljio je sve sigurnosne kriterije te je liofiliziran s 96 % uspješnosti te se kao takav može uključiti u probiotičke proizvode. Less

Abstract (english)

The microbiological diversity of the intestinal microbiota enables the development of key metabolic processes, the balance of the immune system, resistance to pathogenic microorganisms, and many other beneficial effects. If the microbial balance is disturbed, diseases and many health disorders can develop. Lifestyle, diet, stress, medications, and many other factors have a direct impact on this balance. It has been proven that probiotic preparations can have a beneficial effect on the... More disturbed intestinal microbiota, restoring balance, inhibiting pathogenic microorganisms, stimulating the immune system, and exhibiting many other beneficial effects. The probiotics used must meet a number of criteria to ensure the desired action at the target site in the host and to eliminate possible adverse effects. To ensure the above, probiotic characterization is carried out to examine probiotic candidates on a number of criteria and assess their success, and finally probiotic potential. Infant stool is considered a good source of potential probiotics because many studies have shown it to be a place of quality probiotic strains that have proven their beneficial effects on health. In this study, a probiotic characterization of ten lactic acid bacterial isolates from infant stool was performed. The best strain, Lactobacillus plantarum B12, was selected, which showed high survival in simulated conditions of the gastrointestinal system, antimicrobial activity and coaggregation with pathogens, antioxidant activity, and hydrophobicity of the cell surface. L. plantarum B12 met all safety criteria and was lyophilized with 96% success and as such can be included in probiotic products. Less

• Keywords: probiotici; probiotička karaterizacija; stolica dojenčeta; liofilizacija; Lactobacillus
• Keywords (english): probiotics; probiotic characterization; infant; lyophilization; Lactobacillus
• Language: croatian
• URN:NBN: urn:nbn:hr:159:337546
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-10-25 09:26:40