Involvement of DNA sensors in different types of skin injury

master's thesis

• Title (croatian): Uključenost DNA senzora u različitim tipovima ozljede kože
• Author: Klara Čuljak
• Mentor: Vladimir Mrša (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Biochemistry); Zagreb
• Defense date and country: 2021-09-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Skin, the first organ in contact with the environment, plays an important role in protection against pathogens, chemical, mechanical and other injuries. However, superficial skin injuries can disrupt the integrity of the skin and thus lead to the development of numerous diseases that are nowadays widely present. As the concentration of pollutants in the air increases over the years, various skin injuries occur more and more often. Some of the most destructive air pollutants are ozone, ultraviolet radiation, cigarette smoke, particular matter, and many others. According to previous studies, these pollutants are very harmful to human lungs. To determine whether such substances have the same effect on the skin, we established four research models – tape strip injury as a mechanical damage model, acute and chronic ozone, and cigarette smoke exposure as models of chemical damage. According to histological analyses and levels of inflammatory mediators, we found that mechanical damage of the skin is more destructive than chemical one. Also, IFNAR, NLRP3 and STING showed to be promising targets for future research as they revealed a possible protective role in skin injury.

Abstract (croatian)

Koža kao zaštitni organ u svih živih organizama, prva dolazi u doticaj s različitim utjecajima iz okoliša poput patogena, kemijskih, mehaničkih i drugih ozljeda. Površinske ozljede kože mogu utjecati na integritet kože i uzrokovati razvoj brojnih bolesti široko rasprostranjenih u svijetu. Onečišćenje zraka povećava se s godinama i utječe na sve češću pojavu ozljeda kože. Najveći onečišćivači zraka poput ultraljubičastog zračenje, dima cigareta, ozona i mnogih drugih prema brojnim istraživanjima imaju negativan učinak na dišni sustav. Cilj rada bio je utvrditi imaju li navedeni zagađivači i negativan utjecaj na kožu. Uspostavljena su četiri modela istraživanja: mehanička ozljeda kože (ozljeda kože ljepljivom trakom) i kemijska ozljeda kože (akutno izlaganje ozonu, kronično izlaganje ozonu te izlaganje dimu cigareta). Histološkom analizom i mjerenjem razina upalnih medijatora utvrdili smo da je mehaničko oštećenje kože destruktivnije u odnosu na kemijsko oštećenje. DNA senzori IFNAR, NLRP3 i STING pokazali su najveći potencijal u zacjeljivanju kože nakon ozljede. S obzirom na kratko razdoblje istraživanja, navedene DNA senzore i njihovu zaštitnu ulogu treba opsežnije istražiti u budućnosti.

• Keywords: cigarette smoke; DNA sensors; ozone; skin injury; tape strip injury
• Keywords (english): dim cigareta; DNA senzori; ozljeda kože; ozljeda ljepljivom trakom; ozon
• Language: english
• URN:NBN: urn:nbn:hr:159:257347
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-05-26 12:39:02