Primjena rekombinantnih mikroorganizama u proizvodnji biofarmaceutika

undergraduate thesis

• Title (english): Application of recombinant microorganisms in biopharmaceutical production
• Author: Marko Iveša
• Mentor: Blaženka Kos (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2022-06-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Od staničnih linija jajnika kineskog hrčka (CHO) i eukariotskih mikroorganizama do sojeva aktinomiceta i drugih bakterija, u proizvodnji biofarmaceutika uglavnom se koriste rekombinantni organizmi dobiveni metodama genetičkog inženjerstva nakon provedenog sekvencioniranja genoma i provedenih analiza primjenom bioinformatičkih alata. Prije razvoja suvremenih tehnologija, evolucija je bila jedini način da se poboljšaju svojstva radnog mikroorganizma. Broj modela, metoda i instrumenata dostupnih za provođenje genetičkih izmjena domaćina sve je veći te su i mogućnosti dobivanja rekombinantnih radnih mikroorganizama s poželjnim svojstvima poboljšane. U ovom je radu dan pregled primjene metoda genetičkog inženjerstva u kreiranju rekombinantnih organizama namijenjenih biotehnološkoj proizvodnji farmaceutika. Opisani su modelni organizmi, potencijalni radni organizmi koji mogu poslužiti kao platforma zbog povoljnih metaboličkih svojstava ili fizioloških značajki i rekombinantne stanice mikroorganizama i stanične linije biljaka, sisavaca i kukaca. Posebno je obrađena važnost posttranslacijskih modifikacija u proizvodnji biofarmaceutika te proizvodne strategije koje omogućuju primjenu najsusvremenijih metoda na radne organizme time omogučavajući ekonomičnost proizvodnje novih lijekova.

Abstract (english)

From Chinese hamster ovary (CHO) cell lines and eukaryotic microorganisms to actinomycetes and other bacterial strains, the production of biopharmaceuticals mainly uses recombinant organisms obtained by genetic engineering methods after genome sequencing and analysis using bioinformatics tools. Prior to the development of modern technologies, evolution was the only way to improve the properties of the working microorganism. The number of models, methods and instruments available for the genetic modification of hosts is increasing and the possibilities of obtaining recombinant working microorganisms with desirable properties have been improved. Model organisms, potential working organisms that can serve as a platform due to favorable metabolic properties or physiological characteristics, and recombinant microorganisms and plant, mammalian and insect cell lines are described. The importance of post-translational modifications in the production of biopharmaceuticals and production strategies that enable the application of state-of-the-art methods to working organisms, thus enabling the cost-effectiveness of the production of new drugs, is especially discussed.

• Keywords: biofarmaceutici; rekombinantni mikroorganizmi; posttranslacijske modifikacije genetičko inženjerstvo
• Keywords (english): biopharmaceutical production; recombinant microorganisms; posttranslational modifications; genetic engineering
• Language: croatian
• URN:NBN: urn:nbn:hr:159:691493
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-10-05 13:41:36