Formulacija i razvoj višeslojno mikroinkapsuliranoga probiotika s ciljanim učincima na zdravlje

doctoral thesis

• Title (english): Formulation and development of a multi-layered, microencapsulated probiotic with targeted health effects
• Author: Deni Kostelac
• Mentor: Jadranka Frece (mentor)
• Committee member: Ksenija Markov (predsjednik povjerenstva)
• Committee member: Jasna Novak (član povjerenstva)
• Committee member: Marko Gerić (član povjerenstva)
• Committee member: Anita Slavica (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2022-04-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 57 - Biological sciences. Physical anthropology. Biodiversity

Abstract

U ovom radu provedena je izolacija bakterija mliječne kiseline iz mlijeka magarice i kobile, stolice zdravog dojenčeta te je formirana baza izolata kojima je pridodan soj prethodno izoliran iz fermentirane sirutke. Bakterijski sojevi identificirani su i podvrgnuti sustavnoj probiotičkoj karakterizaciji kako bi se izdvojili sojevi s potencijalom u zaštiti zuba i usne šupljine, inhibicijom Helicobacter pylori, protuupalnom i antioksidativnom aktivnošću. Bakterijski izolati iz mlijeka magarice i kobile pokazali su značajnu inhibiciju i koagregaciju s uzročnicima karijesa, gingivitisa i parodontitisa te izraženu protuupalnu aktivnost blokirajući upalnu signalizaciju ljudskih mononuklearnih stanica i proizvodnju proupalnog citokina TNF-α u uvjetima upale. Izolati iz stolice dojenčeta pokazali su inhibitorni potencijal prema patogenoj bakteriji H. pylori dok je Lactobacillus plantarum S1, izoliran iz sirutke, pokazao sposobnost smanjenja oksidativnog stresa u okolini djelujući protektivno na DNA ljudskih mononuklearnih stanica u uvjetima oksidativnog stresa. Navedeni izolati uklopljeni su u višeslojni mikroinkapsulat koji je razvijen korištenjem dvaju neškodljivih polimera alginata i kitozana te je razvijena troslojna kapsula formirana tako da na ciljanom mjestu otpušta sojeve čija je aktivnost potrebna u ustima, želudcu i crijevima.

Abstract (english)

In this study, lactic acid bacteria were isolated from donkey and mare milks and from the feces of a healthy infant, and a base of isolates was formed to which a strain previously isolated from fermented whey was added. The bacterial strains were identified and subjected to systematic probiotic characterization in order to detect the strains with the potential for dental and oral protection, Helicobacter pylori inhibition, anti-inflammatory and antioxidant activities. Bacterial isolates from donkey and mare milk showed significant inhibition and coaggregation with pathogens associated with caries, gingivitis, and periodontitis, and exhibited high anti-inflammatory activity by blocking inflammatory signaling of human mononuclear cells and the production of proinflammatory cytokine TNF-α under inflammatory conditions. The isolates from infant feces showed inhibitory potential against the pathogenic bacterium H. pylori, while Lactobacillus plantarum S1 isolated from whey showed the ability to reduce oxidative stress in the environment by protecting the DNA of human mononuclear cells under oxidative stress. These isolates were incorporated into a multilayer microcapsule developed using two innocuous polymers (alginate and chitosan). This resulted in a three-layer capsule that releases the strains at the target site, whose activity is required in the mouth, stomach, and intestine.

• Keywords: probiotici; Lactobacillus; protuupalno; antioksidativno; mikroinkapsulacija
• Keywords (english): probiotics; Lactobacillus; antiinflammatory; antioxidant; microencapsulation
• Language: croatian
• URN:NBN: urn:nbn:hr:159:929676
• Promotion: 2022
• Study programme: Title: Postgraduate PhD Study in Nutrition; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam (doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam)
• Type of resource: Text
• Extent: 144 str ; 30 cm
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-11-08 10:40:43