Određivanje antimikrobnog djelovanja i minimalne inhibitorne koncentracije hidrofobnih prirodnih niskotemperaturnih eutektičkih otapala

master's thesis

• Title (english): Determination of antimicrobial activity and minimum inhibitory concentration of hydrophobic low temperature natural deep eutectic solvents
• Author: Helena Juričić
• Mentor: Ksenija Markov (mentor)
• Committee member: Kristina Radošević (predsjednik povjerenstva)
• Committee member: Ivana Radojčić Redovniković (član povjerenstva)
• Committee member: Jasna Mrvčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2022-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Razvojem pojačane svijesti o potrebi zaštite okoliša javlja se potreba za razvojem novih tehnologija s manjim utjecajem na okoliš. Jedan od važnih aspekata industrije su otapala, a upravo prirodna niskotemperaturna eutektička otapala (eng. Natural Deep Eutectic Solvent - NADES) pokazuju potencijal kao nova zelena otapala. Prirodna niskotemperaturna eutektička otapala se pripremaju od dvije ili više komponente prirodnog podrijetla. Ukoliko je jedna od komponenti aktivna tvar dobiva se terapeutsko niskotemperaturno eutektičko otapalo (eng. Therapeutic Deep Eutectic Solvents - THEDES). Cilj ovog rada bio je ispitati antimikrobno djelovanje pripremljenih NADES - a i THEDES - a na bakterije Escherichia coli 3014, Pseudomonas aeruginosa 3024, Staphylococcus aureus 3048 i Listeria monocytogenes 3112 te kvasac Candida albicans 86, disk difuzijskom metodom. Na temelju rezultata disk difuzijske metode određivana je minimalna inhibitorna koncentracija. Rezultati su pokazali osjetljivost svih bakterija i kvasca na sve NADES - e. THEDES je također pokazao djelovanje prema svim test mikroorganizmima

Abstract (english)

With increasing awareness of the idea to protect environment, there is need to develop new technologies with less impact. Solvents are important aspect of the industry and Natural Deep Eutectic Solvents (NADES) show high potential as new green solvents. Natural Low Temperature Deep Eutectic Solvents are prepared from two or more natural components. In case one of the components is active pharmaceutical ingredient, solvent is called Therapeutic Low Temperature Deep Eutectic Solvent (THEDES). Aim of this work was to test the antimicrobial effect of the prepared NADESes and THEDESes on the bacteria strains Escherichia coli 3014, Pseudomonas aeruginosa 3024, Staphylococcus aureus 3048, Listeria monocytogenes 3112 and the yeast Candida albicans 86 using the disc diffusion method. Based on the results of the disk diffusion method, minimum inhibitory concentration was observed. Results showed sensitivity of all bacteria and yeast to all NADESes. THEDES also showed activity against all test microorganisms.

• Keywords: prirodno niskotemperaturno eutektičko otapalo; terapeutsko niskotemperaturno eutektičko otapalo; aktivna farmaceutska tvar; bakterije; kvasac
• Keywords (english): Natural Low Temperature Deep Eutectic Solvent; Therapeutic Low Temperaure Deep Eutectic Solvent; Active Pharmaceutical Ingredient; bacteria; yeast
• Language: croatian
• URN:NBN: urn:nbn:hr:159:317578
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-02-27 12:56:48