Biološka evaluacija ferocenskih peptida izvedenih iz ferocenske aminokiseline i hidrofobnih aminokiselina - VAL, LEU I PHE

master's thesis

• Title (english): Biological evaluation of ferrocene peptides derived from ferrocene amino acid and hydrophobic amino acids - VAL, LEU AND PHE
• Author: Ana Dončević
• Mentor: Jasna Mrvčić (mentor)
• Committee member: Monika Kovačević (predsjednik povjerenstva)
• Committee member: Damir Stanzer (član povjerenstva)
• Committee member: Lidija Barišić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Fermentation and Yeast Technology); Zagreb
• Defense date and country: 2022-07-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Moderna medicina u posljednje se vrijeme susreće s vrlo ozbiljnim problemom prekomjerne i nepravilne uporabe antibiotika što rezultira pojavom bakterijskih sojeva s razvijenim mehanizmima obrane te posljedično pojavom antimikrobne rezistencije. Kako bi se riješio ovaj problem znanstvenici razvijaju nove antimikrobne agense koji bi mogli zamijeniti antibiotike te onemogućiti daljnji razvoj antimikrobne rezistencije. Potencijalni antimikrobni agensi su i ferocenski peptidi čija se biološka evaluacija provodi u ovom radu. Antimikrobna aktivnost ferocenskih peptida 1 - 12 provedena je disk difuzijskom metodom, metodom razrjeđivanja bujona te metodom brojanja kolonija dok su za određivanje antioksidacijske aktivnosti korištene FRAP i DPPH metode. Antimikrobnu aktivnost prema test mikroorganizmima: S. aureus, B. subtilis, i P. aeruginosa pokazali su samo spojevi Ac−D-Val−NH−Fn−COOMe (4), Ac−D-Leu−NH−Fn−COOMe (8) i Ac−D-Phe−NH−Fn−COOMe (12). Daljnjim istraživanjem ustanovljeno je da spoj 8 primijenjen u koncentraciji 2 mM uzrokuje inhibiciju rasta test mikroorganizama za jedno decimalno razrjeđenje.

Abstract (english)

Modern medicine is facing a very serious problem namely the excessive and improper use of antibiotics, which leads to the emergence of bacterial strains with developed defense mechanisms and thus to the emergence of antimicrobial resistance. To address this problem, scientists are developing new antimicrobial agents that could replace antibiotics and prevent further development of antimicrobial resistance. Potential antimicrobial agents are ferrocene peptides, and their biological evaluation is examined in this work. The antimicrobial activity of ferrocene peptides 1 - 12 was investigated by the disk diffusion method, the broth dilution method, and the colony counting method, while FRAP and the DPPH method were used to determine the antioxidant activity. Only the compounds Ac−D-Val−NH−Fn−COOMe (4), Ac−D-Leu−NH−Fn−COOMe (8) and Ac−D-Phe−NH−Fn−COOMe (12) showed antimicrobial activity against the test microorganisms: S. aureus, B. subtilis, and P. aeruginosa. Further studies showed that compound 8 at the highest concentration of 2 mM inhibited microbial growth by one decimal dilution.

• Keywords: antimikrobna rezistencija; ferocen; antimikrobna aktivnost; antioksidacijska aktivnost
• Keywords (english): antimicrobial resistance; ferrocene; antimicrobial activity; antioxidant activity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:785048
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-06-02 10:48:49