Utjecaj mutacija proteina Keap1 na interakciju s dipeptidil-peptidazom III

master's thesis

• Title (english): Influence of Keap1 mutations on dipeptidyl peptidase III interaction
• Author: Martina Kolak
• Mentor: Kristina Radošević (mentor)
• Committee member: Igor Stuparević (predsjednik povjerenstva)
• Committee member: Mihaela Matovina (član povjerenstva)
• Committee member: Igor Slivac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2022-07-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Dipeptidil-peptidaza III osim cijepanja dipeptida s N-terminalnog kraja oligopeptida ima i ulogu u regulaciji signalnog puta Keap1-Nrf2. Nrf2 je transkripcijski faktor koji je ključan u odgovoru stanice na oksidacijska oštećenja, a vežući se na protein Keap1 usmjerava se ka ubikvitinaciji i razgradnji. DPP III, kao i Nrf2, sadrži motiv ETGE preko kojeg se ostvaruju interakcije s domenom Kelch proteina Keap1. Na internetskom portalu za genomiku raka cBioPortal nalaze se genomske sekvence mutanata Keap1 pronađene u uzorcima zloćudnih tumora. U ovom radu su proučavane dvije mutacije Keap1 proteina, G333C i G480W, te njihov utjecaj na interakciju s proteinom DPP III. Istraživanje je provedeno primjenom stanične linije HEK293T.

Abstract (english)

Dipeptidyl-peptidase III, in addition to cleaving dipeptides from the N-terminal end of oligopeptides, also plays a role in the regulation of the Keap1-Nrf2 signalling pathway. Nrf2 is a transcription facto that is crucial in the cell response to oxidative damage, and binding to the Keap1 protein directs it toward ubiquitination and degradation. Both Nrf2 and DPP III contain an ETGE motif through which the interactions with the Kelch domain of the Keap1 protein are achieved. The cancer genomics web portal cBioPortal contains genomic sequences of Keap1 mutants found in cancer samples. In this work, two mutations of the Keap1 protein, G333C and G480W, their influence on the interaction with the DPP III protein were studied. The study was performed using the HEK293T cell line.

• Keywords: dipeptidil-peptidaza III; signalni put Keap1-Nrf2; mjesno-specifična mutageneza; kulture stanica; western blot analiza; koimunoprecipitacija
• Keywords (english): dipeptidyl-peptidase III; Keap1-Nrf2 signalling pathway; site-directed mutagenesis; cell culture; Western blot; co-immunoprecipitation
• Language: croatian
• URN:NBN: urn:nbn:hr:159:130918
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-07-21 11:44:04