Ultrazvukom potpomognuta ekstrakcija bioaktivnih molekula ploda pasjeg trna

master's thesis

• Title (english): Ultrasound assisted extraction of bioactive molecules of sea-buckthorn fruit
• Author: Antea Marić
• Mentor: Sandra Pedisić (mentor)
• Committee member: Maja Repajić (predsjednik povjerenstva)
• Committee member: Sandra Balbino (član povjerenstva)
• Committee member: Zoran Zorić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for drying Technologies and monitoring of biologically active compounds); Zagreb
• Defense date and country: 2022-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Cilj ovog istraživanja bio je utvrditi utjecaj ultrazvukom potpomognute ekstrakcije (UAE) primjenom različitih otapala (30 i 70 %-tna vodena otopina etanola, n-heksan i aceton) i vremena ekstrakcije (20, 30 i 40 min) na sadržaj hidrofilnih i lipofilnih bioaktivnih molekula (BM) plodova pasjeg trna (Hippophae rhamnoides) te odrediti njihovu antioksidacijsku aktivnost (AA). Najviši udjeli ukupnih fenolih spojeva određeni su u sorti „Ascola“, a najzastupljeniji spoj bio je flavonol izoramnetin-3-heksozid (69,73 g 100 gˉ¹ s. tv.) te vitamin C (289 mg 100 gˉ¹ s.tv.). Najviši udjeli ukupnih karotenoida određeni su u sorti „Leikora“ (73,61 mg 100 gˉ¹ s. tv.), a najzastupljeniji je bio lutein palmitat (21,85 mg 100 gˉ¹ s. tv.). U sorti „Leikora“ određena je veća AA (2755,95 μmol TE 100 gˉ¹ s. tv.). Bolja učinkovitost UAE hidrofilnih BM postignuta je primjenom 70 %-tne vodene otopine etanola i vremena od 20 min, a lipofilnih primjenom acetona i vremena od 30 min.

Abstract (english)

The aim of this study was to evaluate the influence of ultrasound assisted extraction (UAE) using different solvents (30 and 70% ethanol, n-hexane and acetone) and extraction time (20, 30 and 40 min) on the content of hydrophilic and lipophilic bioactive molecules (BM) of sea-buckthorn fruit (Hippophae rhamnoides) and to determine the antioxidant activity (AA). The higher total phenolic content was found in the variety “Ascola”, and isorhamnetin-3- hexoside (69.73 mg 100 gˉ¹ dm) and vitamin C (289 mg 100 gˉ¹ dm) were the most abundant. The higher carotenoid content was determined in variety “Leicora” (73,61 mg 100 gˉ¹ dm) dominated by lutein palmitate (21.85 mg 100 gˉ¹ dm) as well as higher AA (2755.95 μmol TE 100 gˉ¹ dm). Better UAE efficiency for hydrophilic BM was obtained by using 70% ethanol as solvent and extraction time of 20 min and acetone and 30 min for lipophilic BM.

• Keywords: bioaktivne molekule; plod pasjeg trna; ultrazvukom potpomognuta ekstrakcija; ORAC antioksidacijska aktivnost
• Keywords (english): bioactive molecules; sea-buckthorn fruit; ultrasound assisted extraction; ORAC antioxidant activity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:355692
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-07-21 11:50:59