Primjena spektroskopije nuklearne magnetske rezonancije u metabolomičkim istraživanjima

undergraduate thesis

• Title (english): Application of nuclear magnetic resonance spectroscopy in metabolomic research
• Author: Katarina Paradžik
• Mentor: Lidija Barišić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Organic Chemistry); Zagreb
• Defense date and country: 2023-09-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Spektroskopija nuklearne magnetske rezonancije (NMR), metoda koja se zasniva na magnetskim svoj-stvima jezgara s neparnim atomskim brojem, ima sve veću primjenu u analizi sastava bioloških uzoraka poput urina i krvi. Metabolomika se bavi biokemijskom karakterizacijom metaboloma i njegovih prom-jena uzrokovanih upotrebom lijekova te genetičkim, nutritivnim i okolišnim faktorima. Promjene u sa-stavu metabolita indikator su općeg stanja organizma te je identifikacija i kvantifikacija metabolita klju-čna za predviđanje bolesti, praćenje učinkovitosti terapije te istraživanje utjecaja okolišnih uvjeta na organizam. Visoka reproducibilnost, mogućnost kvantitativne analize te neinvazivnost čine NMR po-godnom metodom za određivanje sastava metaboloma.

Abstract (english)

Nuclear Magnetic Resonance (NMR) spectroscopy, a method based on the magnetic properties of odd-atomic-number nuclei, is increasingly being used to analyse the composition of biological samples such as urine and blood. Metabolomics involves the biochemical characterization of the metabolome and its changes caused by drug usage, genetic, nutritional, and environmental factors. Changes in metabolite composition are indicative of the overall health of an organism, and identification and quantification of metabolites is critical for predicting disease, monitoring the effectiveness of therapy, and studying the effects of environmental conditions on the body. High reproducibility, the ability to perform quantitative analysis and a non-invasive approach make NMR a suitable method for determining the composition of the metabolome.

• Keywords: metabolomika; NMR; MS; izotopi
• Keywords (english): metabolomics; NMR; MS; isotopes
• Language: croatian
• URN:NBN: urn:nbn:hr:159:156273
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-09-22 08:29:11