Učinci prenatalne izloženosti α-cipermetrinu na endokrinu disrupciju reprodukcije i razvoja muških potomaka pokusnih štakora

master's thesis

• Title (english): Effects of prenatal exposure to α-cypermethrin on endocrine disruption of reproduction and development of male laboratory rat offspring
• Author: Lea Biličić
• Mentor: Ksenija Durgo (mentor)
• Committee member: Višnja Gaurina-Srček (predsjednik povjerenstva)
• Committee member: Anja Katić (član povjerenstva)
• Committee member: Jasna Mrvčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2022-07-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Brojni pesticidi djeluju kao hormonski otrovi, koji ovisno o razdoblju izloženosti mogu pridonijeti razvoju reproduktivnih poremećaja kod muškaraca i žena. U ovom radu ispitan je utjecaj potencijalnog hormonskog otrova, piretroida α-cipermetrina, na endokrinu disrupciju reprodukcije i razvoja prenatalno izloženih muških potomaka pokusnih štakora. S ciljem utvrđivanja učinaka prenatalne izloženosti α-cipermetrinu na razvoj muških mladunaca F1 generacije promatrani su: anogenitalni razmak, postojanje areola/bradavica i vanjski znakovi spolnog sazrijevanja. Izmjerene su i tjelesne mase muških mladunaca u pubertetu te mase njihovih testisa. Učinci prenatalne izloženosti α-cipermetrinu na reprodukciju muških mladunaca F1 generacije ispitani su praćenjem parametara endokrine disrupcije prema odgovarajućim OECD protokolima. Također su procijenjene razine testosterona u serumu muških fetusa i razine testosterona i progesterona u serumu muških mladunaca u pubertetu imunokemijskom metodom ELISA.

Abstract (english)

A large number of pesticides act as endocrine disruptors. They could contribute to the development of reproductive disorders in men and women, depending on the period of exposure. In this research we investigated the effect of potential endocrine disruptor, pyrethroid α-cypermethrin, on endocrine disruption of reproduction and the development of prenatally exposed male laboratory rat offspring. In order to determine the effects of prenatal exposure to α-cypermethrin on the development of F1 male offspring anogenital distance, areola/nipple retention and external signs of sexual maturation were monitored. The body mass of pubertal male offspring and mass of their testes were measured. Effects of prenatal exposure to α-cypermethrin on the reproduction of F1 male offspring were investigated by monitoring parameters of endocrine disruption according to the appropriate OECD protocols. The levels of testosterone in foetal serum and testosterone and progesterone in serum of pubertal male offspring were measured using immunochemical method ELISA.

• Keywords: piretroid; hormonski otrov; reprodukcija; razvoj; steroidni hormoni
• Keywords (english): pyrethroid; endocrine disruptor; reproduction; development; steroid hormones
• Language: croatian
• URN:NBN: urn:nbn:hr:159:722629
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-10-06 09:40:47