Pridržavanje mediteranskog načina prehrane i utjecaj na mikrobiotu

master's thesis

• Title (english): Adherence to the Mediterranean diet and impact on gut microbiome
• Author: Matea Škarupka
• Mentor: Jurica Žučko (mentor)
• Committee member: Ivana Rumora Samarin (predsjednik povjerenstva)
• Committee member: Andreja Leboš-Pavunc (član povjerenstva)
• Committee member: Janko Diminić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Section for Bioinformatics); Zagreb
• Defense date and country: 2022-07-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Mediteranska prehrana je način prehrane ljudi s područja mediteranskog bazena, a povezuje se s nizom pozitivnih učinaka na zdravlje. Glavna obilježja ovakvog tipa prehrane su visok udio nezasićenih masnih kiselina, visok unos žitarica, voća i povrća te nizak unos crvenog mesa. Crijevna mikrobiota je zajednica mikroorganizama koji obitavaju u probavnom sustavu domaćina, a sastav mikrobiote podložan je promjenama tijekom života. Prehrana ima velik utjecaj na sastav mikrobiote, stoga je cilj ovog rada bio povezati sastav mikrobiote s usklađenosti prehrane ispitanika s mediteranskom prehranom. U istraživanju je sudjelovalo 59 ispitanika oba spola, koji su ispunili opći upitnik o životnim i prehrambenim navikama te upitnik o usklađenosti prehrane s mediteranskom. Sastav crijevne mikrobiote određen je izolacijom DNA iz uzoraka stolice te sekvenciranjem na Illumina MiSeq uređaju. Podaci dobiveni sekvenciranjem obrađeni su QIIME2 programom. Prema rezultatima ovog rada nije uočena značajna povezanost usklađenosti prehrane s mediteranskom prehranom i sastavom crijevne mikrobiote na promatranoj skupini ispitanika.

Abstract (english)

Mediterranean diet is a diet pattern followed by people from the Mediterranean basin area, and is associated with a number of positive health effects. Main characteristics of this type of diet are high content of unsaturated fatty acids, high intake of cereals, fruits and vegetables and low intake of red meat. Intestinal microbiota is a community of microorganisms that inhabit the hosts digestive system and its composition is susceptible to changes throughout life. Diet has a great influence on the composition of the microbiota, so the aim of this study was to link the composition of microbiota with the compliance of the subjects' diet with the Mediterranean diet. The study involved 59 subjects of both sexes, who completed a general questionnaire on lifestyle and eating habits and a questionnaire on the compliance of diet with the Mediterranean diet. The composition of the intestinal microbiota was determined by isolating DNA from stool samples and sequencing on an Illumina MiSeq device. The data obtained by sequencing was processed using QIIME2. The results of this study show no significant association of compliance with the Mediterranean diet and the composition of the intestinal microbiota in the observed group of subjects.

• Keywords: mikrobiota; sastav mikrobiote; mediteranska prehrana; životni stil
• Keywords (english): microbiota; composition of microbiota; Mediterranean diet; lifestyle
• Language: croatian
• URN:NBN: urn:nbn:hr:159:239166
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-10-06 10:13:08