Aktivnost odabranih oksidoreduktaza u niskotemperaturnim eutektičkim otapalima

undergraduate thesis

• Title (english): Activity of selected oxidoreductased in deep eutectic solvents
• Author: Jasmin Mah'D
• Mentor: Marina Cvjetko Bubalo (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2023-09-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Ovim radom ispitana je aktivnost enzima alkohola dehidrogenaze (ADH) i litičke polisaharidne monooksigenaze (LPMO) u niskotemperaturnim eutektičkim otapalima (engl. Deep Eutectic Solvents, DES). Odabrane su lako dostupne, netoksične i relativno jeftine komponente za izradu niskotemperaturnih eutektičkih otapala prema načelima zelene kemije i održivog razvoja. Nakon sinteze otapala, spektrofotometrijski je ispitana aktivnost ADH u glicin pirofosfatnom puferu pH 9 (GPP) i LPMO u natrijev acetatnom puferu (NaAc) puferu pH 6 u svrhu usporedbe aktivnosti. Potom je aktivnost enzima ispitana u priređenim otapalima istom spektrofotometrijskom metodom uz pufer kao slijepu probu. Svrha rada je bila ispitati primjenu DES-ova te usporediti i eksperimentalno potkrijepiti aktivnost ADH i LPMO u pripremljenim DES-ovima

Abstract (english)

This work investigated the activity of alcohol dehydrogenase (ADH) and lytic polysaccharide monooxygenase enyzme (LPMO) in low-temperature deep eutectic solvents (DES). Easily accessible, non-toxic, and relatively inexpensive components were selected to create the deep eutectic solvents according to the principles of green chemistry and sustainable development. LPMO activity was initially spectrophotometrically tested in NaAc buffer pH 6, while ADH activity was spectrophotometrically tested in glycine pyrophosphate buffer pH 9 for comparison. Then, enzyme activity was tested in prepared solvents using the same method with buffer as a blank. Purpose of the work was to investigate the usage of DES and experimentally substantiate the activity of ADH and LPMO in prepared DES.

• Keywords: aktivnost enzima; alkohol dehidrogenaza; betain; biokataliza; litička polisaharidna monooksigenaza; niskotemperaturna eutektička otapala
• Keywords (english): alcohol dehydrogenase; betaine; enzyme activity; low-temperature deep eutectic solvents; lytic polysaccharide monooxygenases
• Language: croatian
• URN:NBN: urn:nbn:hr:159:136335
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-04-02 08:42:55