Utjecaj hidrofobnih niskotemperaturnih eutektičkih otapala na aktivnost i skladišnu stabilnost lipaza

undergraduate thesis

• Title (english): Hydrophobic deep eutectic solvents impact on lipase activity and storage stability
• Author: Iva Mihaljević
• Mentor: Manuela Panić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2022-09-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Implementacijom načela zelene kemije, koja nudi konkretne smjernice za adaptaciju konvencionalnih i dizajn novih kemijskih produkata i procesa, nastoji se smanjiti ili potpuno ukloniti primjena štetnih i opasnih tvari. Najveći potencijal za zamjenu organskih otapala imaju zelena otapala među kojima se ističu niskotemperaturna eutektička otapala nalazeći svoju primjenu u različitim područjima između kojih je i biokataliza. U ovom radu ispitivan je njihov utjecaj na aktivnost i skladišnu stabilnost različitih lipaza te su u tu svrhu korištena dva hidrofobna niskotemperaturna eutektička otapala (hDES) Ty:C₈ i C₁₂:C₈. Analizom rezultata, najaktivnijom se pokazala lipaza CALB u hDES-u Ty:C₈, dok su najvišu skladišnu stabilnost pokazale lipaza G u hDES-u Ty:C₈ te lipaza AYS u hDES-u C₁₂:C₈. Zaključno, nudeći bolji ekološki otisak hDES-ovi su potencijalna zamjena za primjenu u industriji i eliminaciji organskih otapala kao skladišni medij za lipaze.

Abstract (english)

The implementation of green chemistry principles, which offers specific guidelines for the adaptation of conventional and the design on new chemical products and processes, aims to reduce or eliminate the use of harmful and hazardous substances. Green solvents have the greatest potential to replace organic solvents, among which deep eutectic solvents are highlighted by their application in different areas, including biocatalysis. Their impact on the activity and storage stability of various lipases was studied in this work and two hydrophobic deep eutectic solvents (hDES) Ty:C₈ and C₁₂:C₈ were used for that purpose. Analyzing the results, lipase CALB in hDES Ty:C₈ showed to be the most active, while the highest storage stability showed lipase G in hDES Ty:C₈ and lipase AYS in hDES C₁₂:C₈. In conclusion, offering a better environmental footprint, hDESs are a potential substitute for industrial applications and elimination of organic solvents as a storage medium for lipases.

• Keywords: zelena kemija; eutektička otapala; lipaza; aktivnost; skladišna stabilnost
• Keywords (english): green chemistry; deep eutectic solvents; lipase; activity; storage stability
• Language: croatian
• URN:NBN: urn:nbn:hr:159:738867
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-04-05 09:43:33