Biotehnološka proizvodnja ksilitola iz lignoceluloznih sirovina

undergraduate thesis

• Title (english): Biotechnological production of xylitol from lignocellulosic raw materials
• Author: Valentina Vuradin
• Mentor: Vlatka Petravić Tominac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2022-09-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Ksilitol je šećerni alkohol koji se primjenjuje u prehrambenim i farmaceutskim proizvodima, a najznačajniju ulogu ima kao sladilo. Industrijski proizvedeni ksilitol dobiva se kemijskim putem. Sve se više istražuju biotehnološke metode, koje su povoljnije za okoliš, a koriste široko lignoceluloznu biomasu kao obnovljivu sirovinu. Primjenjuju se odgovarajuće metode predtretmana lignoceluloze sirovina koje prethode biokonverziji u ksilitol. Ksilitol mogu poizvesti različiti mikroorganizmi koji spadaju u kvasce, bakterije i plijesni. Radni mikroorganizmi se međusobno razlikuju po optimalnim uvjetima biosinteze ksilitola. Prema dostupnoj literaturi, sojevi kvasaca Candida i rekombinantne bakterije Eschericia .coli i Corynebacterium glutamicum daju najveće prinose ksilitola. Proizvedeni
ksilitol izdvaja se iz fermentacijske podloge i pročišćava do kristalnog oblika primjenom različitih metoda.

Abstract (english)

Xylitol is a sugar alcohol that is used in food and pharmaceutical products, and its most important role is as a sweetener. Industrially produced xylitol is obtained by chemical methods. Biotechnological methods of xylitol production are increasingly being researched, which are more favorable for the environment and use lignocellulosic biomass as a renewable raw material. The appropriate methods of of lignocellulose pretreatment are applied, which precede the bioconversion into xylitol. Xylitol can be produced by various microorganisms that belong to yeasts, bacteria and molds. Working microorganisms differ from each other in the optimal conditions of xylitol biosynthesis. According to the available literature, Candida yeast strains and recombinant bacteria Eschericia coli and Corynebacterium glutamicum give the highest xylitol yields. The xylitol produced is separated from the fermentation medium and purified to a crystalline form using various methods.

• Keywords: ksilitol; biotehnološka proizvodnja; lignoceluloza; mikroorganizmi; pročišćavanje
• Keywords (english): xylitol; biotechnological production; lignocellulose; microorganisms; purification
• Language: croatian
• URN:NBN: urn:nbn:hr:159:192997
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-04-09 08:38:12