Spektrofluorimetrijsko određivanje koncentracije riboflavina u energetskim pićima

undergraduate thesis

• Title (english): Spectrofluorimetric determination of concentration of riboflavin in energy drinks
• Author: Tea Žugec
• Mentor: Filip Šupljika (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Physical Chemistry and Corrosion); Zagreb
• Defense date and country: 2023-09-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Riboflavin (vitamin B2) je esencijalan vitamin važan za normalno funkcioniranje metaboličkih procesa u organizmima. Zahvaljujući strukturi molekule, riboflavin ima sposobnost fluorescencije. Prirodni izvori riboflavina su hrana biljnog podrijetla, mlijeko i jaja, a može se pronaći i u komercijalnim prehrambenim proizvodima poput energetskih napitaka. Cilj ovog rada bio je odrediti koncentraciju riboflavina u energetskim pićima pomoću spektrofluorimetrijske metode. Spektrofluorimetrija je korištena zbog svoje jednostavnosti i osjetljivosti te zbog fluorescencije riboflavina. Dobiveni rezultati, odnosno izmjerene koncentracije, pokazuju da je spektrofluorimetrijska metoda učinkovita metoda za određivanje koncentracije riboflavina u energetskim pićima. Osim samih izmjerenih koncentracija, koje se u pravilu poklapaju s deklariranim, točnost metode korištene u svrhu određivanja koncentracije riboflavina u energetskim pićima potvrđuju i rezultati provedene metode dodatka standarda.

Abstract (english)

Riboflavin (vitamin B2) is an essential vitamin important for normal functioning of metabolic processes in organisms. Due to the structure of molecule, riboflavin has ability to fluoresce. Natural sources of riboflavin are food of plant origin, milk and eggs, however it can be found in commercial food products such as energy drinks. The aim of this study was to determine concentration of riboflavin in energy drinks using the spectrofluorimetric method. Spectrofluorimetry is used because of its simplicity and sensitivity and because of fluorescence of riboflavin. The results obtained, i.e. the concentrations measured in this study show that the spectrofluorimetric method is an effective method for determination of concentrations of riboflavin in energy drinks. Besides the measured concentrations that generally coincide with declared ones, accuracy of the method used to determine the concentration of riboflavin in energy drinks is confirmed by results of the standard addition method.

• Keywords: riboflavin; spektrofluorimetrija; metoda dodatka standarda
• Keywords (english): riboflavin; spectrofluorimetry; standard addition method
• Language: croatian
• URN:NBN: urn:nbn:hr:159:081184
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-04-16 08:12:44