Uklanjanje fumonizina B1 primjenom otpada riblje industrije

master's thesis

• Title (english): Removal of fumonisin B1 using fishing industry waste
• Author: Andrea Petrinić
• Mentor: Ksenija Markov (mentor)
• Committee member: Nada Vahčić (predsjednik povjerenstva)
• Committee member: Tibor Janči (član povjerenstva)
• Committee member: Jasna Mrvčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2022-12-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Mikotoksini su sekundarni toksični metaboliti filamentoznih plijesni s dokazano negativnim učinkom na zdravlje. Najčešći putevi unosa mikotoksina u organizam podrazumijevaju konzumaciju kontaminirane hrane. Mikotoksini mogu kontaminirati hranu i poljoprivredne proizvode u bilo kojoj fazi proizvodnje, prerade, transporta i skladištenja. Fumonizin B1 (FB1) najčešće se pojavljuje u kukuruzu i proizvodima od kukuruza. Postojeće metode uklanjanja dijele se na fizikalne, kemijske i biološke, no često su nedovoljno efikasne ili nisu praktične za primjenu u industriji. Biosorpcija kao novi način uklanjanja mikotoksina nudi visoku selektivnost i specifičnost, nisku cijenu i ekološku prihvatljivost. Stoga su u ovom radu kao biološki adsorbensi ispitane kosti i ljuske brancina i orade i njihova sposobnost vezanja FB1 u dvije vrste pufera te u simuliranim uvjetima gastrointestinalnog trakta peradi. Sva tri adsorbensa pokazala su dobro vezanje fumonizina B1 u fosfatnom (pH 7) i citratnom puferu (pH 3), dok je u simuliranim uvjetima probavnog trakta peradi ipak zabilježena niža adsorpcija, što ukazuje na potrebu za daljnjim istraživanjima i modifikacijom adsorbensa.

Abstract (english)

Mycotoxins are secondary toxic metabolites of filamentous molds with a proven negative effect on health. The most common ways of introducing mycotoxins into the body involve the consumption of contaminated food. Mycotoxins can contaminate food and agricultural products at any stage of production, processing, transport, and storage. Fumonisin B1 (FB1) is most often found in corn and corn products. Existing removal methods are divided into physical, chemical, and biological, but they are often insufficiently efficient or not practical for industrial application. Biosorption as a new method of mycotoxin removal offers high selectivity and specificity, low cost, and environmental acceptability. Therefore, in this work, the bones and scales of sea bass and sea bream were tested as biological adsorbents and their ability to bind FB1 in two types of buffers and simulated conditions of the gastrointestinal tract of poultry. All three adsorbents showed good binding of fumonisin B1 in phosphate (pH 7) and citrate buffer (pH 3), while in simulated conditions of the digestive tract of poultry lower adsorption was recorded, which indicates the need for further research and modification of the adsorbent.

• Keywords: fumonizin B1; biosorpcija; otpad riblje industrije; hrana za životinje
• Keywords (english): fumonisin B1; biosorption; fish industry waste; animal feed
• Language: croatian
• URN:NBN: urn:nbn:hr:159:783663
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-04-23 12:28:10