Biološki učinak nitrofurantoina i hidrokinona na stanične linije jetre (HEP-G2) i mokraćnog mjehura (T24)

master's thesis

• Title (english): Biological effect of nitrofurantoin and hydroquinone on liver (HEP-G2) and bladder cell LINES (T24)
• Author: Antonio Gagić
• Mentor: Ksenija Durgo (mentor)
• Committee member: Blaženka Kos (predsjednik povjerenstva)
• Committee member: Ana Butorac (član povjerenstva)
• Committee member: Andreja Leboš-Pavunc (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2022-11-02, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Infekcije urinarnog trakta su čest zdravstveni problem koji pogađa veliki broj ljudi, a najčešće su uzrokovane djelovanjem bakterija. Učestalom pojavom bakterijske rezistencije na antibiotike, naglašava se važnost pronalaska novih baktericidnih spojeva ili spojeva koji mogu poboljšati djelovanje antibiotika paralelnom terapijom. U ovom radu je ispitan citotoksični i oksidativni učinka antibiotika nitrofurantoina na stanice hepatocelularnog karcinoma jetre (HepG2), hidrokinona-spoja iz biljnog izvora tenjihove smjese na stanice karcinoma mokraćnog mjehura (T24) u tretmanu bez oporavka i s 24-satnim oporavkom stanica. Također, istražen je i utjecaj nitrofurantoina i hidrokinona na promjene u proteomu T24 stanica tijekom tretmana od 8 h kao prosječnog vremena spavanja pa time i direktne izložnosti ispitivanim terapeuticima. U ovom radu dokazan je antioksidativni učinak istovremenog tretmana T24 stanica nitrofurantoinom i hidrokinonom, a proteomskom analizom dokazan je njihov utjecaj na biosintezu nezasićenih masnih kiselina, održavanje stanične homeostaze te regulaciju polimerizacije i depolimerizacije aktina. Samostalno djelovanje nitrofurantoina utjecalo je na održavanje homeostaze proteina, transkripciju i vezikularni transport u T24 stanicama.

Abstract (english)

Urinary tract infections are a common health problem that affects a large number of people, and are most often caused by bacteria. The frequent appearance of bacterial resistance to antibiotics emphasizes the importance of finding new bactericidal compounds or compounds that can improve the effect of antibiotics with parallel therapy. In this work, the cytotoxic and oxidative effect of the antibiotic nitrofurantoin, naturally occuring compound hydroquinone and the mixture of these two compounds were investigated. As biological test systemhepatocellular liver cancer cells (HepG2), bladder cancer cells (T24) were were used and treatedwithout and with 24-hour cell recovery, respectively. . Also, the influence of nitrofurantoin and hydroquinone on changes in the proteome of T24 cells during treatment of 8 h as an average sleep time was determined. The antioxidant effect of the simultaneous treatment of T24 cells with nitrofurantoin and hydroquinone was proven, and their influence on the biosynthesis of unsaturated fatty acids, the maintenance of cellular homeostasis, and the regulation of polymerization and depolymerization of actin was proven by proteomic analysis. The independent action of nitrofurantoin affected the maintenance of protein homeostasis, transcription and vesicular transport in T24 cells.

• Keywords: nitrofurantoin; hidrokinon; citotoksičnost; oksidacijski učinak; proteom
• Keywords (english): nitrofurantoin; hydroquinone; cytotoxicity; oxidative effect; proteome
• Language: croatian
• URN:NBN: urn:nbn:hr:159:492650
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-04-24 13:02:14