Primjena "Layer by Layer" nanoinkapsulacije Limosilactobacillius sojeva za dostavu probiotičkih stanica in situ

undergraduate thesis

• Title (english): Application of "LbL" nanoencapsulation for enhanced delivery of Limosilactobacillus probiotic cells in situ
• Author: Lucija Vitaliani
• Mentor: Jasna Novak (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2023-09-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Nanoinkapsulacija je suvremeni pristup za ciljanu zaštitu i kontroliranu dostavu probiotičkih sojeva ili pripadajućih postbiotika odnosno bioaktivnih spojeva in situ. Upravo cilj ovog rada je definiranje zaštitnog učinka „layer by layer“ (LbL) nanoinkapsulacije bakterijskih stanica Limosilactobacillus sojeva analizom postotka preživljavanja suhog aktivnog pripravka, neposredno nakon liofilizacije, te mjesec dana tijekom pohrane za pojačanu biodostupnost. Provedena je i nanoinkapsulacija egzopolisharida ovih sojeva koji se istražuju kao postbiotici. Učinkovito formiranje nanokapsula bakterijskih stanica Limosilactobacillus fermentum potvrđeno je mjerenjem zeta potencijala, temeljem kojeg je zabilježena promjena iz pozitivnog u negativni naboj, i obrnuto, tijekom faza nanošenja slojeva elektrolita metodom „sloj po sloj“ nanoinkapsulacije. Postotak preživljavanja nanoinkapsuliranih sojeva Lb. fermentum veći je nakon inkubacije u samom želučanom soku naspram postotka preživljavanja intaktnih sojeva nakon inkubacije u želučanom soku i soku tankog crijeva što upućuje na zaštitnu ulogu „LbL“ nanoinkapsulacije i mogućnost pojačanje biodostupnosti in situ.

Abstract (english)

Nanoencapsulation is a modern approach for targeted protection and controlled in situ delivery of probiotic strains or associated postbiotics or bioactive compounds. The aim of this work is to define the protective effect of "layer by layer" (LbL) nanoencapsulation of bacterial cells of Limosilactobacillus strains by analyzing the survival percentage of the active freeze-dried cells, immediately after lyophilization, and after one month during storage to enhance bioavailability. Nanoencapsulation of the exopolysaccharides of these strains, which are being investigated as postbiotics, was also carried out. The effective formation of nanocapsules of Limosilactobacillus fermentum bacterial cells was confirmed by measuring the zeta potential, based on which the change from positive to negative charge and vice versa was recorded during the stages of layering of electrolyte layers using the "LbL" nanoencapsulation. Survival percentage of nanoencapsulated strains of Lb. fermentum is higher after incubation in gastric juice compared to the survival percentage of intact strains after incubation in gastric juice and small intestine juice, which points to the protective role of "LbL" nanoencapsulation and the possibility of enhancing bioavailability in situ.

• Keywords: biodostupnost; probiotici; „LbL“ nanoinkapsulacija; postbiotici
• Keywords (english): bioavailability; „layer by layer“ nanoencapsulation; probiotics; postbiotics
• Language: croatian
• URN:NBN: urn:nbn:hr:159:344932
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-04-26 08:46:03