Biotehnološka proizvodnja bikaverina i karotenoida pomoću plijesni iz roda Fusarium

undergraduate thesis

• Title (english): Biotechnological production of bikaverine and carotenoids using molds from the genus Fusarium
• Author: Stela Svilović
• Mentor: Nenad Marđetko (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2023-09-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu provedeni su uzgoji plijesni Fusarium oxysporum JCM 9293 i Fusarium verticillioides JCM 23107 s ciljem određivanja koncentracije pigmenata bikaverina i karotenoida koji nastaju kao sekundarni metaboliti uzgoja. Uzgoji su provedeni u hranjivim podlogama sa dva različita izvora ugljika: glukozom i ksilozom te na 3 različite pH-vrijednosti: 8, 6 i 4; u aerobnim uvjetima (250o./min) tijekom 7 dana. Najveća koncentracija bikaverina (0,5827 mol/L) zabilježena je 6. dan uzgoja na podlozi koja je sadržavala glukozu i ksilozu i čija je pH-vrijednost iznosila 6, a radni mikroorganizam je bio Fusarium oxysporum JCM 9293. Najveća koncentracija karotenoida (1,3950 g/L) je izmjerena u uzorcima izoliranim iz biomase nakon 7.-og dana uzgoja na podlozi koja je sadržavala glukozu i ksilozu i čija je pH-vrijednost iznosila 6, a raadni mikroorganizam je također bio Fusarium oxysporum JCM 9293.

Abstract (english)

In this work, molds Fusarium oxysporum JCM 9293 and Fusarium verticillioides JCM 23107 were cultivated with the aim of determining the concentration of bikaverine pigments and carotenoids that are produced as secondary metabolites of cultivation. Cultivations were carried out in media with two different carbon sources: glucose and xylose and at three different pH values: 8, 6 and 4; in aerobic conditions (250 o./min) for 7 days. The highest concentration of bikaverine (0,5827 mol/L) was recorded on the 6th day of cultivation on a medium containing glucose and xylose with pH value of 6 and the woking microorganism - Fusarium oxysporum JCM 9293. The highest concentration of carotenoids (1,3950 g/L) was measured in samples isolated from biomass after the 7th day of cultivation on a medium containing glucose and xylose and whose pH value was 6, with working microorganism Fusarium oxysporum JCM 9293.

• Keywords: Fusarium oxysporum JCM 9293; Fusarium verticillioides JCM 23107; bikaverin; karotenoidi; pigmenti
• Keywords (english): Fusarium oxysporum JCM 9293; Fusarium verticillioides JCM 23107; bikaverine; carotenoids; molds
• Language: croatian
• URN:NBN: urn:nbn:hr:159:811604
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-04-29 09:06:36