Optimizacija parametara uzgoja Euglene gracilis primjenom metode odzivnih površina

undergraduate thesis

• Title (english): Optimization of Euglena gracilis cultivation parameters by response surface methodology
• Author: Ivan Jukić
• Mentor: Tonči Rezić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2014-09-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U budućnosti očekuje se energetska kriza zbog ograničenih fosilnih izvora energije. Jedna od strategija je nadoknaditi dio kapljevitih goriva proizvodnjom biodizela iz lipida mikoralgi. Istraživanje se temelji na proizvodnji lipida iz alge Euglena gracilis te je izveden foto-miksotrofni uzgoj u foto–bioreaktoru s miješalom. Tijekom ovog istraživanja, sastav hranjive podloge i procesni parametri za foto-heterotrofni uzgoj su optimizirani kako bi se definirali adekvatni uvjetiza uzgoj biomase mikroalgi i proizvodnju lipida. Zbog niske cijene i dostupnog izvora ugljika i dušika, kukurzna močevina (CSL) je korištena za rast Euglene gracilis i proizvodnju lipida. Optimizacijom sastava hranjive podloge i uvjeta uzgoja, te korištenjem metode odzivnih površina (RSM) povećana je proizvodnja lipida i biomase do 29 % suhe tvari biomase postupkom u dva stupnja uzgoja u jednom foto–bioreaktoru. Određene optimalne vrijednosti procesenih parametara (koncentracija kukuruzne močevine (CSL 26,4-26,6 g/L ),brzina miješanja (rpm199-283 min-1), aeracija(0,49-0,57 v/v min)) koristiti će se u daljnem istraživanju dobivanja lipida iz biomase mikroalge E. gracilis.

Abstract (english)

In the future, due to limited fossil energy resources, a crisis of energy,is expected. One strategy is to compensate a part of the liquid fuel production of biodiesel from microalgal lipids. The studyis based on the production of lipids from microalgae Euglena gracilis and it was performedphoto-mixotrophic cultivation in the stirred tank photo-bioreactor. During this research, medium composition and operational conditions of photo-bioreactor were optimized in order to define adequate cultivation conditions for algae biomass and lipid production. As low cost and available complex carbon nitrogen source, corn steep liquor was used to promote Euglena gracilis growth and lipid production. Due to optimization of medium composition and cultivation conditions,lipid and biomass production was increased up to 29 % in a two stage cultivation process inside one photo-bioreactor. Thedetermined optimal values of the process parameters (corn steep liquor (CSL 26,4-26,6g/L ), steering speed (rpm 199 -283 min-1), aeration(0,49-0,57 v/v min)) will be used in the following study to obtain lipids from E. gracilis microalgae biomass.

• Keywords: fotomiksotrofni uzgoj; Euglena gracilis; proizvodnja lipida; uvjeti uzgoja; foto-bioreaktor s miješalom; metoda odzivnih površina
• Keywords (english): photo-mixotrophic cultivation; Euglena gracilis; lipid production; cultivation conditions; stirred tank photo-bioreactor; response surface methodology
• Language: croatian
• URN:NBN: urn:nbn:hr:159:872811
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-27 11:43:29