Djelovanje biokonjugata ferocena i derivata 2-tiouracila na HeLa staničnu liniju

master's thesis

• Title (english): Activity of ferrocene bioconjugates with 2-thiouracil derivatives on HeLa cell line
• Author: Iva Logarušić
• Mentor: Kristina Radošević (mentor)
• Mentor: Martina Bagović (pomoć pri izradi, neposredni voditelj)
• Committee member: Senka Djaković (predsjednik povjerenstva)
• Committee member: Višnja Gaurina Srček (član povjerenstva)
• Committee member: Jasmina Lapić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2023-04-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Ferocenski konjugati su organometalni spojevi koji posjeduju različita elektrokemijska i biološka svojstva zbog čega se istražuju i primjenjuju kao novi biološki aktivni spojevi u procesu istraživanja i razvoja lijekova. Budući da veliki broj ferocenskih konjugata pokazuje antitumorski učinak, u ovom radu je ispitana in vitro biološka aktivnost četiri različita biokonjugata ferocena i derivata 2-tiouracila na humanoj tumorskoj staničnoj liniji HeLa određivanjem stanične vijabilnosti i klonogenom analizom. Nadalje, protočnom citometrijom određeno je s kojim se staničnim procesom može povezati zapažena citotoksičnost. Sva četiri ispitana biokonjugata pokazala su inhibitorni učinak na rast i proliferaciju HeLa stanične linije, pri čemu su spojevi 3 i 4 pokazali najizraženiji citotoksični učinak. Inhibitorno djelovanje spojeva 3 i 4 na rast HeLa stanica može se povezati s poticanjem stanične smrti procesom apoptoze. Dobiveni rezultati ukazuju da biokonjugati ferocena i derivata 2-tiouracila imaju potencijal kao antitumorski agensi.

Abstract (english)

Ferrocene conjugates are organometallic compounds that have different electrochemical and biological properties, therefore they have been investigated and used as new biologically active compounds in the process of drug research and development. A large number of ferrocene conjugates show antitumor activity, therefore in this work the in vitro biological activity of four different bioconjugates of ferrocene and 2-thiouracil derivatives was assessed on human tumor cell line HeLa by determination of cell viability and clonogenic analysis. Furthermore, flow cytometry was used to determine which cellular process could be associated with the observed cytotoxicity. All tested bioconjugates showed an inhibitory effect on the growth and proliferation of HeLa cell line and compounds 3 and 4 showed the greatest cytotoxic effect. The inhibitory effect of compounds 3 and 4 on the growth of HeLa cells can be associated with the stimulation of cell death by the process of apoptosis. The obtained results indicate that bioconjugates of ferrocene and 2-thiouracil derivatives have potential as antitumor agents.

• Keywords: biokonjugati ferocena i derivata 2-tiouracila; in vitro biološka aktivnost; tumorska stanična linija HeLa; klonogena analiza; protočna citometrija
• Keywords (english): bioconjugates of ferrocene and 2-thiouracil derivatives; in vitro biological activity; tumor cell line HeLa; clonogenic analysis; flow cytometry
• Language: croatian
• URN:NBN: urn:nbn:hr:159:375783
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-05-14 11:19:46