Izrada upitnika o čimbenicima koji utječu na sastav crijevne mikrobiote i usporedba s eksperimentalno određenim sastavom

master's thesis

• Title (english): The development of a questionnaire on factors affecting the composition of gut microbiota and comparison with experimentally determined composition
• Author: Dražena Čermak
• Mentor: Jurica Žučko (mentor)
• Committee member: Ivana Rumora Samarin (predsjednik povjerenstva)
• Committee member: Andreja Leboš-Pavunc (član povjerenstva)
• Committee member: Antonio Starčević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Section for Bioinformatics); Zagreb
• Defense date and country: 2023-04-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Ljudska mikrobiota u fokusu je istraživanja više no ikad zbog brojnih dokaza o njenoj povezanosti s ljudskim zdravljem i vitalnosti, neovisno o tome radi li se o bakterijama usne šupljine, kože, reproduktivnog sustava, očiju, uha ili gastrointestinalnog sustava. Sastav crijevne mikrobiote ovisi o brojnim biološkim čimbenicima (dob, rasa, spol, bolest) i čimbenicima životnog stila (tjelesna aktivnost, pušenje, alkohol). Bolesti poput dijabetesa, upalnih bolesti crijeva i pretilosti povezuju se s karakterističnim sastavom crijevne mikrobiote, bilo prisutnošću ili odsutnošću nekih rodova i vrsta ili promjenom njihove zastupljenosti. Zbog toga se mikrobiota istražuje kao svojevrsni biomarker zdravlja. Cilj ovog rada bio je istražiti i opisati sastav crijevne mikrobiote s obzirom na različite biološke čimbenike i čimbenike životnog stila. Podaci su prikupljeni anketom i iz bioloških uzoraka stolice ispitanika. Prema rezultatima nije uočena statistički značajna razlika u sastavu crijevne mikrobiote skupina ispitanika s obzirom na dob, spol, bračni status, tip prehrane, tip stolice, naviku pušenja i spavanja

Abstract (english)

The human microbiota is at the focus of researcher because of its connection to health. The composition of gut microbiota is under the influence of many biological factors (age, sex, illness) and lifestyle habits (physical activity, smoking, drinking). Diseases such as diabetes, inflammatory bowel disease and obesity are linked to the specific bacterial composition caused by presence or loss of certain genera or change in their abundance. For this reason, the role of gut microbiota as a biomarker of health should be examined. The aim of this research was to analyse the composition of gut microbiota in regard to biological factors and lifestyle habits. The data was collected through a questionnaire and stool samples of participants. The results show no statistical significance in microbiota composition between groups in regard to age, sex, marital status, type of diet and stool, smoking and sleeping habits.

• Keywords: crijevna mikrobiota; životni stil; zdravlje; QIIME
• Keywords (english): gut microbiome; lifestyle; health; QIIME
• Language: croatian
• URN:NBN: urn:nbn:hr:159:844027
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-05-16 12:42:43